| Texto completo | |
| Autor(es): |
Bankole, Victor Oloruntoba
[1, 2]
;
Osungunna, Michael Oluwole
[1, 2]
;
Fernandes Souza, Claudia Regina
[1]
;
Salvador, Sergio Luiz
[1]
;
Oliveira, Wanderley Pereira
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Av Cafe S-N, Ribeirao Preto 14040903, SP - Brazil
[2] Obafemi Awolowo Univ, Fac Pharm, Dept Pharmaceut, Ife 220005 - Nigeria
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | AAPS PHARMSCITECH; v. 21, n. 5 MAY 18 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
This work aims to improve the functionality of Rosmarinus officinalis L. (rosemary) polyphenols by encapsulation in an optimized proliposome formulation. A 2(3) Box-Wilson central composite design (CCD) was employed to determine lone and interaction effects of composition variables on moisture content (X-p); water activity (A(w)); concentration and retention of rosemary polyphenols-rosmarinic acid (ROA), carnosol (CAR), and carnosic acid (CNA); and recovery of spray-dried proliposomes (SDP). Processing conditions which generate proliposomes with optimum physicochemical properties were determined by multi-response analysis (desirability approach). Antioxidant and antifungal activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH center dot) sequestering and minimum inhibitory concentration (MIC)/minimum fungicidal concentration (MFC) assays, respectively. SDP exhibited high polyphenol retention, ranging from 62.0 to 100.0% w/w, showing dependence on composition variables and polyphenol lipophilicity. SDP recovery ranged from 20.1 to 45.8%, with X-p and A(w) of 1.7 +/- 0.14-2.5 +/- 0.23% w/w and 0.30 +/- 0.004-0.47 +/- 0.003, respectively, evidencing product with good chemical and microbiological stability. Optimum liposomal composition was determined, namely, lipid concentration (4.26% w/w), lyophilized extract (LE) concentration (4.48% w/w), and drying aid:(lipid+extract) ratio (7.55% w/w) on wet basis. Relative errors between experimental and predicted values for SDP properties showed concurrence for all responses except CAR retention, being 22% lower. SDP showed high antioxidant activity with IC50 of 9.2 +/- 0.2 mu g/mL, superior to results obtained for LE (10.8 mu g/mL) and butylated hydroxytoluene (BHT), a synthetic antioxidant (12.5 mu g/mL). MIC and MFC against Candida albicans (ATCC1023) were 312.5 mu g/mL and 1250 mu g/mL, respectively, a moderate antimicrobial activity for phytochemical-based products. SDP is shown as a veritable tool to encapsulate hydrophilic and lipophilic rosemary polyphenols generating a product with optimal physicochemical and biological properties. (AU) | |
| Processo FAPESP: | 14/15905-1 - Encapsulação de óleos essenciais em carreadores lipídicos: desenvolvimento tecnológico e aplicações |
| Beneficiário: | Wanderley Pereira Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/26069-0 - Encapsulação de óleos essenciais para uso em formulações farmacêuticas com atividade antimicrobiana |
| Beneficiário: | Wanderley Pereira Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/10333-1 - Microencapsulação e nanoencapsulação de ativos naturais empregando tecnologias de secagem |
| Beneficiário: | Cláudia Regina Fernandes de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |