| Texto completo | |
| Autor(es): Mostrar menos - |
da Cunha, Flavia Franco
[1]
;
Andrade-Oliveira, Vinicius
[2]
;
de Almeida, Danilo Candido
[1]
;
da Silva, Tamiris Borges
[1]
;
de Souza Breda, Cristiane Naffah
[2]
;
Cruz, Mario Costa
[2]
;
Faquim-Mauro, Eliana L.
[3]
;
Cenedeze, Marcos Antonio
[1]
;
Hiyane, Meire Ioshie
[2]
;
Pacheco-Silva, Alvaro
[1, 4]
;
Cavinato, Regiane Aparecida
[1]
;
Torrecilhas, Ana Claudia
[5]
;
Saraiva Camara, Niels Olsen
[1, 2]
Número total de Autores: 13
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Nefrol, Rua Pedro Toledo 669, BR-04039032 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Imunol, Ave Prof Lineu Prestes 1730, ICB IV, BR-05508000 Sao Paulo - Brazil
[3] Inst Butantan, Lab Imunopatol, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil
[4] Hosp Israelita Albert Einstein, Ave Albert Einstein, BR-62705652 Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Ciencias Farmaceut, Rua Sao Nicolau 210, BR-09913030 Sao Paulo - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CELLS; v. 9, n. 4 APR 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Mesenchymal stromal cells (MSCs) can generate immunological tolerance due to their regulatory activity in many immune cells. Extracellular vesicles (EVs) release is a pivotal mechanism by which MSCs exert their actions. In this study, we evaluate whether mesenchymal stromal cell extracellular vesicles (MSC-EVs) can modulate T cell response. MSCs were expanded and EVs were obtained by differential ultracentrifugation of the supernatant. The incorporation of MSC-EVs by T cells was detected by confocal microscopy. Expression of surface markers was detected by flow cytometry or CytoFLEX and cytokines were detected by RT-PCR, FACS and confocal microscopy and a miRNA PCR array was performed. We demonstrated that MSC-EVs were incorporated by lymphocytes in vitro and decreased T cell proliferation and Th1 differentiation. Interestingly, in Th1 polarization, MSC-EVs increased Foxp3 expression and generated a subpopulation of IFN-gamma(+)/Foxp3(+)T cells with suppressive capacity. A differential expression profile of miRNAs in MSC-EVs-treated Th1 cells was seen, and also a modulation of one of their target genes, TGFbR2. MSC-EVs altered the metabolism of Th1-differentiated T cells, suggesting the involvement of the TGF-beta pathway in this metabolic modulation. The addition of MSC-EVs in vivo, in an OVA immunization model, generated cells Foxp3(+). Thus, our findings suggest that MSC-EVs are able to specifically modulate activated T cells at an alternative regulatory profile by miRNAs and metabolism shifting. (AU) | |
| Processo FAPESP: | 17/05264-7 - Metabolismo celular, microbiota e sistema imune: novos paradigmas na fisiopatologia das doenças renais |
| Beneficiário: | Niels Olsen Saraiva Câmara |
| Linha de fomento: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/08479-7 - EMU concedido no processo 2017/05264-7: analisador de fluxo extracelular Seahorse XFe96 |
| Beneficiário: | Niels Olsen Saraiva Câmara |
| Linha de fomento: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 14/07390-1 - Microvesículas derivadas de células estromais mesenquimais como indutoras de tolerância imunológica |
| Beneficiário: | Flavia Franco da Cunha |
| Linha de fomento: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 16/13029-5 - Papel dos miRNAs na tolerância imunológica mediada por células T reguladoras |
| Beneficiário: | Flavia Franco da Cunha |
| Linha de fomento: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |