Aluminum, at an environmental concentration, associated with acidic pH and high water temperature, causes impairment of sperm quality in the freshwater teleost Astyanax altiparanae (Teleostei: Characidae)

Autor(es)

Silva Pinheiro, Joao Paulo ^[1] ; de Assis, Cecilia Bertacini ^[1] ; Sanches, Eduardo Antonio ^[2] ; Moreira, Renata Guimaraes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Metab & Reprod Aquat Organisms LAMEROA, Inst Biosci, Dept Physiol, Matao St, 14 Lane, 101 Room 220, Cidade Univ, Sao Paulo, SP - Brazil

^[2] Univ Estadual Paulista, Fishery Engn Course, Nelson Brihi Badur Ave 430, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Pollution; v. 262, JUL 2020.
• Citações Web of Science: 0

Resumo

Given the toxicity of metals, including aluminum (Al), and the effects of water temperature on ectotherms, we investigated the individual or association effect of these variables (Al + acidic pH + temperature changes) on sperm quality of Astyanax altiparanae. Mature males were divided into nine experimental groups based on the combination of each of three water temperatures (20, 25, and 30 degrees C) with neutral and acidic pH values (7.0 and 5.5, respectively) with or without 0.5 mg L-1 Al. The fish were subjected to subacute, semi-static exposure and at 24 and 96 h were evaluated for seminal parameters: (1) pH; (2) osmolality; (3) sperm concentration; (4) sperm morphology; (5) sperm kinetics; and (6) sperm ultrastructure. At 30 degrees C, Al caused a reduction in osmolality (24 and 96 h) and sperm concentration (24 h). When analysing sperm kinetics (30 s post-activation), Al caused a reduction in total motility at all temperatures (24 h), and when this exposure time was longer (96 h), both acidic pH and Al addition to the water caused sperm motility reduction. By analysing curvilinear velocity (VCL) 30 s after sperm activation (24 and 96 h), the acidic pH caused a reduction in sperm movement at 20 and 30 degrees C, but at 25 degrees C Al triggered this reduction. Finally, Al in the water caused ultrastructural changes in the sperm head, midpiece, and flagella regardless of water temperature. Also, it was found that the combination of Al at 30 degrees C caused a reduction in sperm head area while at 20 degrees C, Al triggered a reduction in the midpiece area. Therefore, acidity influenced some A. altiparanae sperm parameters but Al in the water accentuated these effects on seminal quality, especially seminal osmolality and sperm concentration, kinetics, and ultrastructure. This toxicity was also influenced by changes in water temperature. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 16/08770-8 - Avaliação da qualidade seminal, mutagênese e genotoxicidade em Astyanax altiparanae (Teleostei, Characidae) expostos ao alumínio em diferentes temperaturas de água
• Beneficiário: João Paulo Silva Pinheiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado