| Texto completo | |
| Autor(es): Mostrar menos - |
Campos, Rafael
[1, 2]
;
Jacintho, Felipe Fernandes
[1]
;
Britto-Junior, Jose
[1]
;
Monica, Fabiola Z.
[1]
;
Oliveira Justo, Alberto Fernando
[1]
;
Pupo, Andre Sampaio
[3]
;
Moreno, Ronilson Agnaldo
[1]
;
de Souza, Valeria Barbosa
[1]
;
Schenka, Andre Almeida
[1]
;
Antunes, Edson
[1]
;
De Nucci, Gilberto
[4, 1]
Número total de Autores: 11
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, UNICAMP, Campinas - Brazil
[2] Ceara State Univ UECE, Super Inst Biomed Sci, BR-60714903 Fortaleza, Ceara - Brazil
[3] UNESP Sao Paulo State Univ, Inst Biosci Botucatu, Dept Pharmacol, Botucatu, SP - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 233, JUL 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
The role of endothelium in the electrical-field stimulation (EFS)-induced contractions of Chelonoidis carbonaria aorta was investigated. Contractions were evaluated in the presence and absence of L-NAME (100 mu M), tetrodotoxin (1 mu M), phentolamine (10 and 100 mu M), phenoxybenzamine (1 and 10 mu M), prazosin (100 mu M), idazoxan (100 mu M), atropine (10 mu M), D-tubocurarine (10 mu M) or indomethacin (10 mu M). EFS-induced contraction was also carried out in endothelium-denuded rings. EFS-induced contraction was investigated by the sandwich assay. Concentration curves to endothelin-1 (0.1-100 nM) and U46619 (0.001-100 mu M) were also constructed to calculate both Emax and EC50. EFS at 16 Hz contracted Chelonoidis aorta, which was almost abolished by the endothelium removal. The addition of L-NAME increased the EFS response (2.0 +/- 0.4 and 8.3 +/- 1.9 mN). In L-NAME treated aortic rings, tetrodotoxin did not change the EFS-response (5.1 +/- 1.8 and 4.9 +/- 1.7 mN). Indomethacin, atropine and d-tubucurarine also did not affect the EFS-response. Phentolamine at 10 mu M did not change the EFS-induced contraction; however, at 100 mu M, reduced it (3.9 +/- 1 and 1.9 +/- 0.3 mN). Prazosin and idazoxan did not change EFS-induced contractions. Phenoxybenzamine at 1 reduced by 76% (9.6 +/- 3.4 and 2.3 +/- 0.8 mN) and at 10 mu M by 90% the EFS response. Immunohistochemistry identified tyrosine hydroxylase in the endothelium and brain, whereas S100 protein was found only in brain. In conclusion, endothelium modulates EFS-induced contractions in Chelonoidis aortic rings and this modulation may be due to endothelium-derived catecholamines, possibly dopamine. (AU) | |
| Processo FAPESP: | 18/24971-9 - Relevância fisiológica das catecolaminas produzidas pelo endotélio |
| Beneficiário: | Felipe Fernandes Jacintho |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 16/04731-8 - Atividade antineoplásica e anti-célula tronco tumoral in vitro e in vivo de sinvastatina, óleo de Copaifera reticulata Ducke e nanopartículas inéditas de óxido de grafeno e de magnetita, em uso isolado ou combinado, em linhagens de carcinoma mamário |
| Beneficiário: | André Almeida Schenka |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/09539-8 - Identificação e caracterização farmacológica, eletrofisiológica e morfológica de novo canal de sódio tetrodotoxina-resistente acoplado à musculatura lisa de corpo cavernoso de cobra |
| Beneficiário: | Alberto Fernando Oliveira Justo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |