Angiotensin II Regulates Proliferation and Function of Stem Cells of Apical Papilla

Autor(es)

Pizzatto, Lais Nicolay ^[1] ; Meneses, Claudia C. B. ^[1] ; Diniz, Elisa A. ^[1] ; Dionisio, Thiago J. ^[2] ; Santos, Carlos Ferreira ^[2] ; Sipert, Carla R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Dent Sch Bauru, Dept Biol Sci, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 46, n. 6, p. 810-817, JUN 2020.
• Citações Web of Science: 0

Resumo

Introduction: Stem cells of apical papilla (SCAP) may be affected by inflammatory mediators released by activation with lipopolysaccharide (LPS) from infected pulpal cavities of necrotic immature teeth. Therefore, this study aimed to investigate the presence of a local reninangiotensin system (RAS) and the role of angiotensin II (Ang II) on the modulation of SCAP in vitro. Methods: Primary cultures of SCAP were incubated with LPS (0.1-10 mg/mL) for cell viability and quantification of the chemokine CCL2. Components of RAS were searched by gene expression of angiotensinogen (AGTN), angiotensin converting enzyme (ACE), renin, angiotensin receptor 1 (AT1) and 2 (AT2), and Mas receptor. Ang II was investigated in SCAP supernatants. Immunofluorescence was used to detect AGTN and AT1. Next, cells were treated with Ang II for viability/proliferation assessment, quantification of CCL2 and interleukin 6, and mineralization assay. Data were evaluated by analysis of variance using Tukey post hoc comparisons or the Student t test. P values,.05 were considered to be significant. Results: LPS increased CCL2 production at 1 and 10 mg/mL. The gene expression of AGTN, renin, ACE, and AT1 was detected, but only ACE was increased by LPS. Ang II peptide was found in SCAP supernatants but unaltered by LPS. Both AGTN and AT1 proteins were detected by immunostaining. Ang II significantly induced SCAP proliferation, increased CCL2 production, down-regulated IL-6 release, and reduced the SCAP mineralization rate. Conclusions: A local RAS was found at the apical papilla, and Ang II was able to modulate SCAP function in vitro. (AU)

• Processo FAPESP: 17/01737-8 - Papel de endocanabinóides na modulação de células de papila apical humana in vitro
• Beneficiário: Claudia Caroline Bosio Meneses
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/16473-3 - Estudo do papel de angiotensina II na diferenciação de células de papila apical humana in vitro
• Beneficiário: Laís Nicolay Pizzatto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/13944-5 - Papel de mediadores inflamatórios na modulação de células de papila apical humana in vitro
• Beneficiário: Carla Renata Sipert
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Temático