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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy

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Autor(es):
Dare, Regina G. [1] ; Costa, Ana [2, 3] ; Nakamura, V, Celso ; Truiti, Maria C. T. [4] ; Ximenes, Valdecir F. [5] ; Lautenschlager, Sueli O. S. [4] ; Sarmento, Bruno [2, 3, 6]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] State Univ Maringa UEM, Dept Pharm, BR-87020900 Maringa, Parana - Brazil
[2] Univ Porto, i3S Inst Res & Innovat Hlth, Rua Alfredo Allen 208, P-4200135 Porto - Portugal
[3] Univ Porto, INEB Inst Biomed Engn, Rua Alfredo Allen 208, P-4200135 Porto - Portugal
[4] Nakamura, Celso, V, State Univ Maringa UEM, Dept Pharm, BR-87020900 Maringa, Parana - Brazil
[5] Univ Sao Paulo, Fac Sci, Dept Chem, UNESP, BR-17033360 Bauru, SP - Brazil
[6] IINFACTS Inst Res & Adv Training Hlth Sci & Techn, CESPU, Rua Cent Gandra 1317, P-4585116 Gandra - Portugal
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: International Journal of Pharmaceutics; v. 582, MAY 30 2020.
Citações Web of Science: 0
Resumo

Excessive exposure to solar radiation induces injurious effects on human skin. Our previous study evidenced that protocatechuic acid (P0) and ethyl protocatechuate (P2) act against photodamage and photoaging. The present study aimed to develop solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for topical delivery of P0 or P2, as a strategy for photoprotection. Lipid nanoparticles exhibited mean particle size, polydispersity index, zeta potential and association efficiency between 200 and 400 nm, 0.160 to 0.460, -2.2 to -5.2 mV, and 60% to 80%, respectively. The formulations were stable for 3 months when stored at 4 degrees C and 25 degrees C/60% RH. SLNs/NLCs-P0 showed minor cytotoxicity effects compared with SLNs/NLCs-P2, in HaCat (keratinocytes) and HFF-1 (fibroblasts) cell lines. Additionally, bare NLCs exhibited less cytotoxicity effect, compared with bare SLNs. NLCs exhibited a controlled in vitro release of P0 and P2, and were able to protect the compounds against UVB degradation. Ex vivo permeability study showed that NLCs modulated P0 and P2 retention profiles on human skin layers. Furthermore, histological analysis of skin showed that NLCs-P0 did not cause morphological alterations, while NLCs-P2 showed a potential irritation effect in the skin structure. Based on these results, NLCs were considered a potential dermatological nanocarrier for P0 delivery. (AU)

Processo FAPESP: 14/50926-0 - INCT 2014: biodiversidade e produtos naturais
Beneficiário:Vanderlan da Silva Bolzani
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático