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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of inactivated Bordetella pertussis as a delivery system for the immunization of mice with Pneumococcal Surface Antigen A

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Autor(es):
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Castro, Julia T. [1] ; Oliveira, Giuliana S. [1] ; Nishigasako, Melissa A. [1] ; Debrie, Anne-Sophie [2] ; Miyaji, Eliane N. [1] ; Soares-Schanoski, Alessandra [1] ; Akamatsu, Milena A. [3] ; Locht, Camille [2] ; Ho, Paulo L. [3] ; Mielcarek, Nathalie [2] ; Oliveira, Maria Leonor S. [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Bacteriol, Sao Paulo, SP - Brazil
[2] Univ Lille, Inst Pasteur Lille, CIIL Ctr Infect & Immun Lille, CNRS, INSERM, CHU Lille, U1019, UMR 8204, Lille - France
[3] Inst Butantan, Secao Vacinas Aerob, Div Bioind, Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 15, n. 1 JAN 16 2020.
Citações Web of Science: 1
Resumo

Pneumococcal Surface Protein A (PspA) has been successfully tested as vaccine candidate against Streptococcus pneumoniae infections. Vaccines able to induce PspA-specific antibodies and Th1 cytokines usually provide protection in mice. We have shown that the whole cell pertussis vaccine (wP) or components from acellular pertussis vaccines, such as Pertussis Toxin or Filamentous Hemagglutinin (FHA), are good adjuvants to PspA, suggesting that combined pertussis-PspA vaccines would be interesting strategies against the two infections. Here, we evaluated the potential of wP as a delivery vector to PspA. Bordetella pertussis strains producing a PspA from clade 4 (PspA4Pro) fused to the N-terminal region of FHA (Fha44) were constructed and inactivated with formaldehyde for the production of wP(PspA4Pro). Subcutaneous immunization of mice with wP(PspA4Pro) induced low levels of anti-PspA4 IgG, even after 3 doses, and did not protect against a lethal pneumococcal challenge. Prime-boost strategies using wP(PspA4Pro) and PspA4Pro showed that there was no advantage in using the wP(PspA4Pro) vaccine. Immunization of mice with purified PspA4Pro induced higher levels of antibodies and protection against pneumococcal infection than the prime-boost strategies. Finally, purified Fha44:PspA4Pro induced high levels of anti-PspA4-Pro IgG, but no protection, suggesting that the antibodies induced by the fusion protein were not directed to protective epitopes. (AU)

Processo FAPESP: 17/01992-8 - Ação adjuvante da Hemaglutinina filamentosa (FHA) de Bordetella pertussis em fusão com o antígeno PspA de Streptococcus pneumoniae
Beneficiário:Melissa Akemi Nishigasako
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 16/13134-3 - Vacinas recombinantes contra Streptococcus pneumoniae e Bordetella pertussis
Beneficiário:Maria Leonor Sarno de Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 16/17258-9 - Bordetella pertussis e seus componentes como sistemas de entrega e adjuvantes para o desenvolvimento de vacinas contra Streptococcus pneumoniae
Beneficiário:Júlia Tavares de Castro
Linha de fomento: Bolsas no Brasil - Doutorado Direto