Oxidative stress, biotransformation enzymes and histopathological alterations in Nile tilapia (Oreochromis niloticus) exposed to new and used automotive lubricant oil

Autor(es)

Freitas, Juliane Silberschmidt ^[1] ; Boscolo Pereira, Thiago Scremin ^{[2, 3]} ; Pereira Boscolo, Camila Nomura ^[2] ; Garcia, Mariana Navarro ^[4] ; de Oliveira Ribeiro, Ciro Alberto ^[5] ; de Almeida, Eduardo Alves ^[6]

Afiliação do(s) autor(es)

^[1] Minas Gerais State Univ UEMG, Dept Biol, R Ver Geraldo Moises da Silva S-N, BR-38302192 Ituiutaba, MG - Brazil

^[2] UNIRP Univ Ctr Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

^[3] FACERES Med Sch, FACERES Morfofunct Lab, Sao Jose Do Rio Preto, SP - Brazil

^[4] Univ Estadual Paulista, IBILCE, UNESP, Dept Chem & Environm Sci, R Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Fed Parana, Dept Cell Biol, Ctr Politecn 19031, BR-81531900 Curitiba, Parana - Brazil

^[6] Fundacao Univ Reg Blumenau, Dept Nat Sci, Av Antonio da Veiga 140, BR-89030903 Blumenau, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 234, AUG 2020.
• Citações Web of Science: 0

Resumo

Lubricant oils are among oil-based products that are not fully consumed during its use, thereby producing non-biodegradable residues which can cause contamination of natural systems. This study evaluated the toxicity of new and used lubricating oil (0.01 and 0.1 mL L-1) in adult Nile tilapia (Oreochromis niloticus), by assessing the effects on oxidative stress, biotransformation enzymes (liver and gills), and histopathological alterations on hepatic and pancreatic tissues after 3 and 7 days of exposure. Results showed that 3-days exposure to 0.1 mL L-1 of used and new lubricating oil increased the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver of O. niloticus, respectively. In gills, catalase (CAT) was decreased in fish exposed to 0.1 mL L-1 of non-used oil after 3 days, but pronounced increases in CAT was detected after 7 days-exposure to both new and used oil. Shorter exposure to both concentrations of new and used oil also raised glutathione-S-transferase activity (GST) in gills. Ethoxyresorufin-O-deethylase (EROD) was induced in liver of fish exposed to 0.1 mL L-1 of used oil after 3 and 7 days, however a reduced response of this enzyme was detected in gills of animals from both oil treatments. In vitro analysis showed that hepatic EROD was inhibited by lubricating oil exposures, with more pronounced responses in treatments containing used oil. Hepatic lesions, such as cytoplasmic vacuolization, nuclei abnormally, changes in hepatocytes shape, steatosis, cholestasis, eosinophilic inclusions and necrosis were mainly increased by 7 days exposure to used lubricating oil at higher concentration. (AU)

• Processo FAPESP: 06/03873-1 - Estudo de variações bioquímicas em tilápias, como biomarcadores de contaminação ambiental
• Beneficiário: Eduardo Alves de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/13377-6 - Avaliação dos efeitos ecotoxicológicos de agroquímicos utilizados em pastagens e em culturas de cana-de-açúcar sobre anfíbios.
• Beneficiário: Juliane Silberschmidt Freitas
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado