Occurrence and abundance of clinically relevant antimicrobial resistance genes in environmental samples after the Brumadinho dam disaster, Brazil

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Rueda Furlan, Joao Pedro ^[1] ; Rodrigues dos Santos, Lucas David ^[1] ; Silva Moretto, Jessica Aparecida ^[1] ; Ramos, Micaela Santana ^[1] ; Lage Gallo, Inara Fernanda ^[1] ; Dias Alves, Georgia de Assis ^[1] ; Paulelli, Ana Carolina ^[1] ; de Souza Rocha, Cecilia Cristina ^[1] ; Cesila, Cibele Aparecida ^[1] ; Gallimberti, Matheus ^[1] ; Devoz, Paula Picoli ^[1] ; Barbosa Junior, Fernando ^[1] ; Stehling, Eliana Guedes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 726, JUL 15 2020.
• Citações Web of Science: 2

Resumo

On January 25th 2019, the structure damming a pond containing ore mining wastes and iron burst at Brumadinho City, Brazil. About 11.7 million m(3) of a tailings-mud mixture was released from the dam, causing destruction along 300 km of the Paraopeba River toward the Sao Francisco River. The environments with a high content of metals may provide a suitable environment for horizontal gene transfer, including antimicrobial resistance genes (ARGs). Therefore, this study aimed to detect and quantify clinically relevant ARGs in environmental samples after the Brumadinho dam disaster. Soil, sediment, and water samples were collected within 300 km of the Brumadinho dam disaster at unaffected and affected sites. Physical-chemical parameters of water samples were measured. Total DNA was extracted and 65 clinically relevant ARGs were researched by PCR. The most prevalent ARGs were selected for real-time quantitative PCR analysis. The average of the physical-chemical parameters was higher in the affected sites when compared to the unaffected sites, especially turbidity, concentration of Fe and Al. A total of 387 amplicons from 29 ARGs were detected, which confer resistance to beta-lactams, quinolones, aminoglycosides, tetracyclines, sulphonamides, phenicols, macrolides, glycopeptides, and polymyxins, including extended-spectrum beta-lactamases-encoding genes, and mcr-7.1. The sul1 gene had higher total concentrations than bla(TEM), tetB and qnrB in the environmental samples, and the diversity and abundance of ARGs increased at the sites affected by the Brumadinho dam disaster. Therefore, we point out that the contamination by the Brumadinho dam disaster tailings resulted in an increase in the amount and abundance of ARGs in the environment. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/01890-3 - Estudo do perfil de resistência, virulência e epidemiológico de Escherichia coli isoladas do meio ambiente
• Beneficiário: João Pedro Rueda Furlan
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/19539-0 - Caracterização molecular de isolados de Escherichia coli provenientes do meio ambiente
• Beneficiário: Eliana Guedes Stehling
• Modalidade de apoio: Auxílio à Pesquisa - Regular