High Level of Resistance to Antimicrobials and Heavy Metals in Multidrug-Resistant Pseudomonas sp. Isolated from Water Sources

Autor(es)

Ramos, Micaela Santana ^[1] ; Rueda Furlan, Joao Pedro ^[1] ; Lage Gallo, Inara Fernanda ^[1] ; Rodrigues dos Santos, Lucas David ^[1] ; de Campos, Tatiana Amabile ^[2] ; Savazzi, Eduardo Angelino ^[3] ; Stehling, Eliana Guedes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Brasilia UnB, Inst Ciencias Biol, Dept Biol Celular, Brasilia, DF - Brazil

^[3] Companhia Ambiental Estado Sao Paulo CETESB, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Current Microbiology; v. 77, n. 10 MAY 2020.
• Citações Web of Science: 0

Resumo

Antimicrobial-resistant bacteria (ARB) carrying antimicrobial resistance genes (ARGs) have been increasingly detected in water sources. Pseudomonas sp. are opportunistic pathogens commonly reported in water samples and different antimicrobial resistance mechanisms have been described in Pseudomonas sp., producing multidrug-resistant (MDR) phenotype. Besides, the presence of heavy metal resistance genes (HMRGs) may select ARB, which is worrying. Therefore, this study aimed to characterize the genotypes of Pseudomonas sp. isolated from different water sources. Water samples (i.e., rivers, streams, lakes and sewage treatment plants) were collected from different cities in Brazil. The bacterial identification was performed by sequencing the 16S rDNA and the resistance profile to antimicrobials and heavy metals were determined by minimum inhibitory concentration (MIC). Several ARGs, HMRGs, and plasmids were researched by PCR and the amplicons were sequenced for confirmation. A total of 23 Pseudomonas sp. isolates were obtained and identified as Pseudomonas saponiphila, Pseudomonas hunanensis, Pseudomonas aeruginosa, and Pseudomonas asiatica. These isolates presented high MICs to antimicrobials and heavy metals, being the great majority (n = 21; 91%) classified as MDR. Different clinically important ARGs were detected, such as bla(GES), qnrS, qepA, tetB, aac(3 `)-IIa, and ant(2 `')-Ia. The czcA gene was the only HMRG detected and no plasmids were found. To the best of our knowledge, this is the first report of the world of P. saponiphila carrying ARGs (i.e., bla(GES), qnrS, aac(3 `)-IIa, tetB) and QepA-producing P. hunanensis and the first time of P. saponiphila, P. asiatica, and P. hunanensis in Brazil. (AU)

• Processo FAPESP: 18/01890-3 - Estudo do perfil de resistência, virulência e epidemiológico de Escherichia coli isoladas do meio ambiente
• Beneficiário: João Pedro Rueda Furlan
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/19539-0 - Caracterização molecular de isolados de Escherichia coli provenientes do meio ambiente
• Beneficiário: Eliana Guedes Stehling
• Modalidade de apoio: Auxílio à Pesquisa - Regular