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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Small heat shock protein genes are developmentally regulated during stress and non-stress conditions in Blastocladiella emersonii

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Autor(es):
Georg, Raphaela Castro [1, 2] ; Oshiquiri, Leticia Harumi [2] ; Barbosa-Filho, Jomal Rodrigues [2] ; Gomes, Suely Lopes [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil
[2] Univ Fed Goias, Inst Ciencias Biol, Dept Bioquim & Biol Mol, Goiania, Go - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FUNGAL BIOLOGY; v. 124, n. 5, p. 482-489, MAY 2020.
Citações Web of Science: 0
Resumo

Small heat shock proteins (sHsps) are molecular chaperones of low molecular weight involved in an early association with misfolded proteins. In response to heat shock, B. emersonii induces the synthesis of a number of proteins. As sHsps are still poorly studied in B. emersonii and in fungi overall, the aim of this work was to carry out a in-depth characterization of sHsps during B. emersonni life cycle, as well as in response to thermal stress. We verified a strong induction of the hsp17 gene in cells exposed to heat shock both in germination and sporulation stages, and that Hsp17 protein levels show the same pattern of variation of its mRNA. Unlike hsp17 and hsp30, hsp16 gene is not significantly induced during heat shock, in germination or sporulation cells. However, at normal temperatures, the hsp16 gene presents high mRNA levels in sporulation cells, whereas the hsp30 gene presents high mRNA levels in germination cells. Interestingly, heat shock mRNA levels for hsp17 and hsp30 genes are 10 times higher in germination cells than in sporulation cells. Thus, our data show that the expression of these sHsp genes is quite distinct, both under normal temperature as during heat shock. (C) 2020 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 12/19960-1 - Aspectos moleculares da biologia do fungo aquático Blastocladiella emersonii
Beneficiário:Suely Lopes Gomes
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/20571-6 - International Symposium on Fungal Stress - ISFUS
Beneficiário:Drauzio Eduardo Naretto Rangel
Modalidade de apoio: Auxílio Organização - Reunião Científica