Small heat shock protein genes are developmentally regulated during stress and non-stress conditions in Blastocladiella emersonii

Author(s)

Georg, Raphaela Castro ^{[1, 2]} ; Oshiquiri, Leticia Harumi ^[2] ; Barbosa-Filho, Jomal Rodrigues ^[2] ; Gomes, Suely Lopes ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil

^[2] Univ Fed Goias, Inst Ciencias Biol, Dept Bioquim & Biol Mol, Goiania, Go - Brazil

• Document type: Journal article
• Source: FUNGAL BIOLOGY; v. 124, n. 5, p. 482-489, MAY 2020.
• Web of Science Citations: 0

Abstract

Small heat shock proteins (sHsps) are molecular chaperones of low molecular weight involved in an early association with misfolded proteins. In response to heat shock, B. emersonii induces the synthesis of a number of proteins. As sHsps are still poorly studied in B. emersonii and in fungi overall, the aim of this work was to carry out a in-depth characterization of sHsps during B. emersonni life cycle, as well as in response to thermal stress. We verified a strong induction of the hsp17 gene in cells exposed to heat shock both in germination and sporulation stages, and that Hsp17 protein levels show the same pattern of variation of its mRNA. Unlike hsp17 and hsp30, hsp16 gene is not significantly induced during heat shock, in germination or sporulation cells. However, at normal temperatures, the hsp16 gene presents high mRNA levels in sporulation cells, whereas the hsp30 gene presents high mRNA levels in germination cells. Interestingly, heat shock mRNA levels for hsp17 and hsp30 genes are 10 times higher in germination cells than in sporulation cells. Thus, our data show that the expression of these sHsp genes is quite distinct, both under normal temperature as during heat shock. (C) 2020 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/19960-1 - Molecular aspects of the biology of the aquatic fungus Blastocladiella emersonii
• Grantee: Suely Lopes Gomes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/20571-6 - International Symposium on Fungal Stress - ISFUS
• Grantee: Drauzio Eduardo Naretto Rangel
• Support Opportunities: Organization Grants - Scientific Meeting