Proteome alterations associated with the oleic acid and cis-9, trans-11 conjugated linoleic acid content in bovine skeletal muscle

Autor(es):Mostrar menos -

Poleti, Mirele D. ^[1] ; Regitano, Luciana C. A. ^[2] ; Souza, Gustavo H. M. F. ^[3] ; Cesar, Aline S. M. ^[4] ; Simas, Rosineide C. ^[4] ; Silva-Vignato, Barbara ^[4] ; Montenegro, Horacio ^[4] ; Pertille, Fabio ^[4] ; Balieiro, Julio C. C. ^[5] ; Cameron, Luiz C. ^{[6, 7]} ; Eler, Joanir P. ^[1] ; Coutinho, Luiz L. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Anim Sci & Food Engn FZEA, BR-13635900 Pirassununga, SP - Brazil

^[2] Embrapa Pecuaria Sudeste, BR-13560970 Sao Carlos, SP - Brazil

^[3] MS Applicat & Dev Lab Waters Corp, BR-06455020 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Av Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

^[5] Univ Sao Paulo, Sch Vet Med & Anim Sci FMVZ, BR-13635900 Pirassununga, SP - Brazil

^[6] Fed Univ State Rio de Janeiro UNIRIO, Lab Prot Biochem, BR-22290255 Rio De Janeiro, RJ - Brazil

^[7] Brazil Olymp Comm, Dept Biochem & Sport, Olymp Lab, Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOMICS; v. 222, JUN 30 2020.
• Citações Web of Science: 2

Resumo

Oleic acid (OA) and cis-9, trans-11 conjugated linoleic acid (c9t11-CLA) are fatty acids found in beef with beneficial effects in human health. This study investigated differentially abundant proteins (DAPs) in skeletal muscle of bovines with extreme values of OA, and c9t11-CLA. For each one of the fatty acids, twenty muscle samples were divided into two groups (N = 10\_High; N = 10\_Low) and analyzed by high definition mass spectrometry. We identified 103 and 133 DAPs between the groups for each fatty acid. We found 64 and 45 up-regulated and 39 and 68 down-regulated proteins for OA and c9t11-CLA, respectively. Comparative analysis between proteomic and transcriptomic data revealed eight and ten genes with a consistent between mRNA expression levels and protein abundance for OA and c9t11-CLA, respectively. Unconventional myosin-Id (MYO1D), mineralocorticoid receptor (NR3C2), geranylgeranyl transferase type-2 subunit-alpha (RABGGTA), and uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA) were found as putative candidate proteins for OA content. Fatty acid synthase (FASN), tubulin alpha-4A chain (TUBA4A), vinculin (VCL), NADH dehydrogenase 1 alpha subcomplex 5 (NDUFA5), and prefoldin subunit 6 (PFDN6) for c9t11-CLA. Our findings contribute to a deeper understanding of the molecular mechanisms behind the regulation of the OA and c9t11-CLA content in cattle skeletal muscle. Significance: Questions about the association between meat intake and disease incidence in humans has driven animal scientist to pursue a better understanding of the biological processes associated with differences in the intramuscular fat composition. The beneficial effects of oleic acid and conjugated linoleic acid in human health have been demonstrated by improving the immune system and preventing atherosclerosis, different types of cancers, hypertension, and diabetes. Previous genome-wide association and gene expression studies identified genomic regions and differentially expressed genes associated with the fatty acid profile in skeletal muscle. In this work, differences were evaluated at the protein level. The use of a label-free quantitative proteomic approach, compared with muscle transcriptome results obtained by RNA-sequencing, allowed us to earn new in-sights into the variability in fatty acid deposition in skeletal muscle of farm animals. This study opens new avenues to explore the effect of the fatty acids in the skeletal muscle of livestock animals, which is associated with nutritional values of the meat, and perhaps to understand the mechanisms correlated with metabolic diseases in other species. (AU)

• Processo FAPESP: 13/21017-9 - Estudo de proteínas associadas a fenótipos de qualidade da carne em bovinos da raça Nelore
• Beneficiário: Mirele Daiana Poleti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/23638-8 - Bases moleculares da qualidade da carne em bovinos da raça Nelore
• Beneficiário: Luciana Correia de Almeida Regitano
• Modalidade de apoio: Auxílio à Pesquisa - Temático