| Texto completo | |
| Autor(es): |
Fischer, Reinhard
[1]
Número total de Autores: 1
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| Afiliação do(s) autor(es): | [1] Karlsruhe Inst Technol KIT, Inst Appl Biosci, Dept Microbiol, South Campus, Fritz Haber Weg 4, D-76131 Karlsruhe - Germany
Número total de Afiliações: 1
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| Tipo de documento: | Artigo Científico |
| Fonte: | FUNGAL BIOLOGY; v. 124, n. 5, p. 447-457, MAY 2020. |
| Citações Web of Science: | 1 |
| Resumo | |
A large proportion of fungal genomes are under the control of light. Most fungi employ complex light sensing systems, consisting of red-, blue-, and in some cases green-light photoreceptors. Here we studied the light response in Aspergillus nidulans. In a genetic screen, followed by whole-genome sequencing we identified a global regulator, which appears to be involved in chromatin structure modification. We therefore named the protein RlcA (regulator of light sensing and chromatin remodeling). The protein comprises a nuclear localization signal, a PHD (plant homeodomain) finger, a TFSII (found in the central region of the transcription elongation factor S-II), and a SPOC domain (Spen paralog and ortholog C-terminal domain). In the mutant, where light-controlled genes were constitutively active, the SPOC domain is missing. RlcA localized to the nucleus and interacted with the phytochrome FphA. The PHD-finger domain probably binds to trimethylated lysine 4 of histone H3, whereas the TFSII domain binds RNA polymerase II. The SPOC domain could mediate interaction with a global repressor protein. In the mutant, repressor recruitment would be hindered, whereas in the wild type repressor release would be induced after light stimulation. Our results add another layer of complexity to light sensing in filamentous fungi. (C) 2020 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 18/20571-6 - International Symposium on Fungal Stress - ISFUS |
| Beneficiário: | Drauzio Eduardo Naretto Rangel |
| Modalidade de apoio: | Auxílio Organização - Reunião Científica |