A Halotolerant Endo-1,4-beta-Xylanase fromAspergillus clavatuswith Potential Application for Agroindustrial Residues Saccharification

Autor(es)

Pasin, Thiago Machado ^{[1, 2]} ; Santos Salgado, Jose Carlos ^[3] ; de Almeida Scarcella, Ana Silvia ^[1] ; de Oliveira, Tassio Brito ^[4] ; de Lucas, Rosymar Coutinho ^{[4, 1]} ; Cereia, Mariana ^[4] ; Rosa, Jose Cesar ^{[5, 6]} ; Ward, Richard John ^[3] ; Buckeridge, Marcos Silveira ^[7] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Indiana Univ Purdue Univ, Dept Chem & Chem Biol, Indianapolis, IN 46202 - USA

^[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Prot Chem Ctr, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Dept Mol & Cell Biol & Pathogen Bioagents, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil

^[7] Univ Sao Paulo, Inst Biosci, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 191, n. 3, p. 1111-1126, JUL 2020.
• Citações Web of Science: 1

Resumo

The use of non-potable water (such as seawater) is an attractive alternative for water intensive processes such as biomass pretreatment and saccharification steps in the production of biochemicals and biofuels. Identification and application of halotolerant enzymes compatible with high-salt conditions may reduce the energy needed for non-potable water treatment and decrease waste treatment costs. Here we present the biochemical properties of a halotolerant endo-1,4-beta-xylanase produced byAspergillus clavatusin submerged fermentation, using paper sludge (XPS) and sugarcane bagasse (XSCB), and its potential application in the hydrolysis of agroindustrial residues. The peptide mass fingerprint and amino acid sequencing of the XPS and XSCB enzymes showed primary structure similarities with an endo-1,4-beta-xylanase fromAspergillus clavatus(XYNA\_ASPCL). Both enzyme preparations presented good thermal stability at 50 degrees C and were stable over a wide range of pH andV(max)up to 2450 U/mg for XPS. XPS and XSCB were almost fully stable even after 24 h of incubation in the presence of up to 3 M NaCl, and their activity were not affected by 500 mM NaCl. Both enzyme preparations were capable of hydrolyzing paper sludge and sugarcane bagasse to release reducing sugars. These characteristics make this xylanase attractive to be used in the hydrolysis of biomass, particularly with brackish water or seawater. (AU)

• Processo FAPESP: 17/09000-4 - Efeitos da temperatura e da deficiência hídrica, em cenário climático futuro, na comunidade de fungos em solo tropical e na seleção de linhagens produtoras de óxido nitroso
• Beneficiário: Tássio Brito de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/52322-3 - Bioprospecção de fungos filamentosos produtores de holoenzimas com aplicação em biorefinaria
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular