| Texto completo | |
| Autor(es): |
da Silva, Marcelo S.
[1]
;
Vitarelli, Marcela O.
[1]
;
Souza, Bruno F.
[1]
;
Elias, Maria Carolina
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Lab Ciclo Celular, BR-05503900 Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GENES; v. 11, n. 5 MAY 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Single-celled eukaryote genomes predominantly replicate through multiple origins. Although origin usage during the S-phase has been elucidated in some of these organisms, few studies have comparatively approached this dynamic. Here, we developed a user-friendly website able to calculate the length of the cell cycle phases for any organism. Next, using a formula developed by our group, we showed a comparative analysis among the minimum number of replication origins (MO) required to duplicate an entire chromosome within the S-phase duration in trypanosomatids (Trypanosoma cruzi, Leishmania major, and Trypanosoma brucei) and yeasts (Saccharomyces cerevisiae and Schizosaccharomyces pombe). Using the data obtained by our analysis, it was possible to predict the MO required in a situation of replication stress. Also, our findings allow establishing a threshold for the number of origins, which serves as a parameter for genome approaches that map origins. Moreover, our data suggest that when compared to yeasts, trypanosomatids use much more origins than the minimum needed. This is the first time a comparative analysis of the minimum number of origins has been successfully applied. These data may provide new insight into the understanding of the replication mechanism and a new methodological framework for studying single-celled eukaryote genomes. (AU) | |
| Processo FAPESP: | 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular |
| Beneficiário: | Hugo Aguirre Armelin |
| Linha de fomento: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 14/24170-5 - Dinâmica da replicação do DNA em Trypanosoma cruzi: caracterização do licenciamento e da taxa de replicação |
| Beneficiário: | Marcelo Santos da Silva |
| Linha de fomento: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites? |
| Beneficiário: | Maria Carolina Quartim Barbosa Elias Sabbaga |
| Linha de fomento: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/18719-2 - Análise estrutural do complexo de reconhecimento de origens de replicação (ORC) em Trypanosoma brucei utilizando cryo-electron microscopy e single-particle analysis |
| Beneficiário: | Marcelo Santos da Silva |
| Linha de fomento: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 17/07693-2 - Dinâmica funcional da proteína Orc1b durante o ciclo de vida de Trypanosoma |
| Beneficiário: | Marcela de Oliveira Vitarelli |
| Linha de fomento: | Bolsas no Brasil - Doutorado |