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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative Analysis of the Minimum Number of Replication Origins in Trypanosomatids and Yeasts

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Author(s):
da Silva, Marcelo S. [1] ; Vitarelli, Marcela O. [1] ; Souza, Bruno F. [1] ; Elias, Maria Carolina [1]
Total Authors: 4
Affiliation:
[1] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Lab Ciclo Celular, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: GENES; v. 11, n. 5 MAY 2020.
Web of Science Citations: 0
Abstract

Single-celled eukaryote genomes predominantly replicate through multiple origins. Although origin usage during the S-phase has been elucidated in some of these organisms, few studies have comparatively approached this dynamic. Here, we developed a user-friendly website able to calculate the length of the cell cycle phases for any organism. Next, using a formula developed by our group, we showed a comparative analysis among the minimum number of replication origins (MO) required to duplicate an entire chromosome within the S-phase duration in trypanosomatids (Trypanosoma cruzi, Leishmania major, and Trypanosoma brucei) and yeasts (Saccharomyces cerevisiae and Schizosaccharomyces pombe). Using the data obtained by our analysis, it was possible to predict the MO required in a situation of replication stress. Also, our findings allow establishing a threshold for the number of origins, which serves as a parameter for genome approaches that map origins. Moreover, our data suggest that when compared to yeasts, trypanosomatids use much more origins than the minimum needed. This is the first time a comparative analysis of the minimum number of origins has been successfully applied. These data may provide new insight into the understanding of the replication mechanism and a new methodological framework for studying single-celled eukaryote genomes. (AU)

FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 14/24170-5 - DNA replication dynamics in Trypanosoma cruzi: licensing and replication rate characterization
Grantee:Marcelo Santos da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites?
Grantee:Maria Carolina Quartim Barbosa Elias Sabbaga
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/18719-2 - Structural analysis of the origin recognition complex (ORC) in Trypanosoma brucei using cryo-electron microscopy and single-particle analysis
Grantee:Marcelo Santos da Silva
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 17/07693-2 - Functional dynamics of Orc1b protein during the life cycle of Trypanosoma
Grantee:Marcela de Oliveira Vitarelli
Support type: Scholarships in Brazil - Doctorate