Miniaturization of liquid chromatography coupled to mass spectrometry. 2. Achievements on modern instrumentation for miniaturized liquid chromatography coupled to mass spectrometry

Autor(es)

Vargas Medina, Deyber Arley ^[1] ; Soares Maciel, Edvaldo Vasconcelos ^[1] ; de Toffoli, Ana Lucia ^[1] ; Lancas, Fernando Mauro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: TRAC-TRENDS IN ANALYTICAL CHEMISTRY; v. 128, JUL 2020.
• Citações Web of Science: 0

Resumo

Capillary and nano liquid chromatography coupled to mass spectrometry has recently become one of the primary analytical techniques in many science areas of maior interest, including omics, food security, environmental surveillance, biomedicine, among others. This interest relays on their economical, environmental and analytical advantages, such as low volumes of reagents and sample requirement, gains on chromatographic performance, and enhanced MS sensitivity. In recent decades significant achievements in instrumentation have been reported, which are increasing the scope of applications based on this technique. Nowadays, miniaturized LC instruments with different setups are commercially available or being in-house developed. In brief, this work summarizes the main characteristics of them, underscoring the main parts of the benchtop systems. For this reason, specific topics are discussing the main advantages and challenges of pumping, injection, and detection modules as well as tubing and connectors. Moreover, considering the higher compatibility between miniaturized LC and MS, this coupling provides higher ionization efficiency improving the analytical results, when compared to conventional LC-MS. Consequently, the essential characteristics of atmospheric pressure ionization for miniaturized LC-MS were highlighted, and a discussion regarding the current state-of-art about the use of electron ionization is also presented. In short, this work underlines the main factors related to instrumental advancements and its consequent influence on analytical methods based on miniaturized LC coupled to mass spectrometry. (C) 2020 Published by Elsevier B.V. (AU)

• Processo FAPESP: 17/02147-0 - Cromatografia líquida em uma gota e seu acoplamento com espectrometria de massas: estratégias instrumentais, desenvolvimento de materiais, automatização e aplicações analíticas
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/15462-5 - Emprego do grafeno como material sorvente em técnicas miniaturizadas de preparo de amostra.
• Beneficiário: Bruno Henrique Fumes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/07347-9 - Desenvolvimento de novos materiais e dispositivos para técnicas miniaturizadas de preparo de amostras na área de segurança alimentar
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/22724-7 - Novas estratégias instrumentais no desenvolvimento de métodos analíticos automatizados e miniaturizados para a determinação de compostos orgânicos mediante cromatografia líquida acoplada a espectrometria de massas
• Beneficiário: Deyber Arley Vargas Medina
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado