Effects of sulforaphane on the oxidative response, apoptosis, and the transcriptional profile of human stomach mucosa cells in vitro

Autor(es)

Santos, Patrick Wellington ^[1] ; Thomazela Machado, Ana Rita ^[1] ; De Grandis, Rone ^[2] ; Ribeiro, Diego Luis ^[2] ; Tuttis, Katiuska ^[2] ; Morselli, Marco ^[3] ; Aissa, Alexandre Ferro ^[1] ; Pellegrini, Matteo ^[3] ; Greggi Antunes, Lusania Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Sao Paulo, SP - Brazil

^[3] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90024 - USA

• Tipo de documento: Artigo de Revisão
• Fonte: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 854, JUN-JUL 2020.
• Citações Web of Science: 0

Resumo

Oxidative stress is a critical factor in the pathogenesis of several gastrointestinal diseases. Sulforaphane (SFN), a bioactive compound found in cruciferous vegetables, activates the redox-sensitive nuclear erythroid 2-related factor 2 (NRF2). In addition to its protective role, SFN exerts cytotoxic effects on cancer cells. However, there is a lack of information concerning the toxicity of SFN in normal cells. We investigated the effects of SFN on cell viability, antioxidant defenses, and gene expression in human stomach mucosa cells (MNP01). SFN reduced ROS formation and protected the cells against induced oxidative stress but high concentrations increased apoptosis. An intermediate SFN concentration (8 mu M) was chosen for RNA sequencing studies. We observed upregulation of genes of the NRF2 (antioxidant) pathway, the DNA damage response, and apoptosis signaling; whereas SFN downregulated cell cycle and DNA repair pathway genes. SFN may be cytoprotective at low concentrations and cytotoxic at high concentrations. (AU)

• Processo FAPESP: 16/14703-1 - Influência do sulforafano, um inibidor de histonas desacetilases, sobre a instabilidade genômica e mecanismos epigenéticos em linhagens celulares humanas
• Beneficiário: Patrick Wellington da Silva dos Santos
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/21561-1 - Efeitos do sulforafano nos padrões de metilação do DNA e análise do transcriptoma em células tumorais e não tumorais
• Beneficiário: Patrick Wellington da Silva dos Santos
• Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 17/24576-0 - Efeitos dos nutracêuticos sulforafano, dissulfeto de dialila e vitamina D em linhagens tumorais humanas: Avaliação de citotoxicidade, genotoxicidade, migração celular, alterações epigenéticas e expressão gênica
• Beneficiário: Lusânia Maria Greggi Antunes
• Linha de fomento: Auxílio à Pesquisa - Regular