Plasma Lipid Profile Reveals Plasmalogens as Potential Biomarkers for Colon Cancer Screening

Autor(es):Mostrar menos -

Fernandes, Anna Maria A. P. ^[1] ; Messias, Marcia C. F. ^[1] ; Duarte, Gustavo H. B. ^[2] ; de Santis, Gabrielle K. D. ^[1] ; Mecatti, Giovana C. ^[1] ; Porcari, Andreia M. ^[1] ; Murgu, Michael ^[3] ; Simionato, Ana Valeria C. ^{[4, 5]} ; Rocha, Thalita ^[1] ; Martinez, Carlos A. R. ^[1] ; Carvalho, Patricia O. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Francisco Univ, Lab Multidisciplinary Res, BR-12916900 Braganca Paulista, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem IQ, UNICAMP, BR-13083970 Campinas, SP - Brazil

^[3] Waters Technol Brazil, Luiz Barssotti Applicat Lab, BR-06455020 Barueri, SP - Brazil

^[4] Natl Inst Sci & Technol Bioanalyt, UNICAMPCampinas, IQ, BR-13083970 Sao Paulo - Brazil

^[5] Univ Estadual Campinas, IQ, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLITES; v. 10, n. 6 JUN 2020.
• Citações Web of Science: 0

Resumo

In this era of precision medicine, there is an increasingly urgent need for highly sensitive tests for detecting tumors such as colon cancer (CC), a silent disease where the first symptoms may take 10-15 years to appear. Mass spectrometry-based lipidomics is an emerging tool for such clinical diagnosis. We used ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry operating in high energy collision spectral acquisition mode (MSE) mode (UPLC-QTOF-MSE) and gas chromatography (GC) to investigate differences between the plasmatic lipidic composition of CC patients and control (CTR) subjects. Key enzymes in lipidic metabolism were investigated using immuno-based detection assays. Our partial least squares discriminant analysis (PLS-DA) resulted in a suitable discrimination between CTR and CC plasma samples. Forty-two statistically significant discriminating lipids were putatively identified. Ether lipids showed a prominent presence and accordingly, a decrease in glyceronephosphate O-acyltransferase (GNPAT) enzyme activity was found. A receiver operating characteristic (ROC) curve built for three plasmalogens of phosphatidylserine (PS), named PS(P-36:1), PS(P-38:3) and PS(P-40:5), presented an area under the curve (AUC) of 0.998, and sensitivity and specificity of 100 and 85.7% respectively. These results show significant differences in CC patients' plasma lipid composition that may be useful in discriminating them from CTR individuals with a special role for plasmalogens. (AU)

• Processo FAPESP: 17/25017-4 - Microextração em fase sólida e cromatografia gasosa na análise lipidômica em plasma de pacientes com câncer colorretal
• Beneficiário: Gabrielle Kristine Doratiotto de Santis
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/13317-6 - Estudo lipidômico aplicado ao Câncer de Reto: mecanismos bioquímicos-moleculares e avaliação da resposta à terapia neoadjuvante
• Beneficiário: Patrícia de Oliveira Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular