| Full text | |
| Author(s): Show less - |
Fernandes, Anna Maria A. P.
[1]
;
Messias, Marcia C. F.
[1]
;
Duarte, Gustavo H. B.
[2]
;
de Santis, Gabrielle K. D.
[1]
;
Mecatti, Giovana C.
[1]
;
Porcari, Andreia M.
[1]
;
Murgu, Michael
[3]
;
Simionato, Ana Valeria C.
[4, 5]
;
Rocha, Thalita
[1]
;
Martinez, Carlos A. R.
[1]
;
Carvalho, Patricia O.
[1]
Total Authors: 11
|
| Affiliation: | [1] Sao Francisco Univ, Lab Multidisciplinary Res, BR-12916900 Braganca Paulista, SP - Brazil
[2] Univ Estadual Campinas, Inst Chem IQ, UNICAMP, BR-13083970 Campinas, SP - Brazil
[3] Waters Technol Brazil, Luiz Barssotti Applicat Lab, BR-06455020 Barueri, SP - Brazil
[4] Natl Inst Sci & Technol Bioanalyt, UNICAMPCampinas, IQ, BR-13083970 Sao Paulo - Brazil
[5] Univ Estadual Campinas, IQ, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | METABOLITES; v. 10, n. 6 JUN 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
In this era of precision medicine, there is an increasingly urgent need for highly sensitive tests for detecting tumors such as colon cancer (CC), a silent disease where the first symptoms may take 10-15 years to appear. Mass spectrometry-based lipidomics is an emerging tool for such clinical diagnosis. We used ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry operating in high energy collision spectral acquisition mode (MSE) mode (UPLC-QTOF-MSE) and gas chromatography (GC) to investigate differences between the plasmatic lipidic composition of CC patients and control (CTR) subjects. Key enzymes in lipidic metabolism were investigated using immuno-based detection assays. Our partial least squares discriminant analysis (PLS-DA) resulted in a suitable discrimination between CTR and CC plasma samples. Forty-two statistically significant discriminating lipids were putatively identified. Ether lipids showed a prominent presence and accordingly, a decrease in glyceronephosphate O-acyltransferase (GNPAT) enzyme activity was found. A receiver operating characteristic (ROC) curve built for three plasmalogens of phosphatidylserine (PS), named PS(P-36:1), PS(P-38:3) and PS(P-40:5), presented an area under the curve (AUC) of 0.998, and sensitivity and specificity of 100 and 85.7% respectively. These results show significant differences in CC patients' plasma lipid composition that may be useful in discriminating them from CTR individuals with a special role for plasmalogens. (AU) | |
| FAPESP's process: | 17/25017-4 - Solid-phase microextraction and gas chromatography in the lipidomic analysis of colorectal cancer patients' plasma |
| Grantee: | Gabrielle Kristine Doratiotto de Santis |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 18/13317-6 - A lipidomics study applied to Rectal Cancer: biochemical-molecular mechanisms and assessment of the response to neoadjuvant therapy |
| Grantee: | Patrícia de Oliveira Carvalho |
| Support Opportunities: | Regular Research Grants |