Multiple resistance ofPlasmopara viticolato QoI and CAA fungicides in Brazil

Autor(es)

Santos, Ricardo F. ^[1] ; Fraaije, Bart A. ^[2] ; Garrido, Lucas da R. ^[3] ; Monteiro-Vitorello, Claudia B. ^[4] ; Amorim, Lilian ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Plant Pathol & Nematol, Luiz de Queiroz Coll Agr, BR-13418900 Piracicaba, SP - Brazil

^[2] Natl Inst Agr Bot, Cambridge - England

^[3] Embrapa Grape & Wine, Bento Goncalves - Brazil

^[4] Univ Sao Paulo, Dept Genet, Luiz de Queiroz Coll Agr, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT PATHOLOGY; v. 69, n. 9 AUG 2020.
• Citações Web of Science: 0

Resumo

Grapevine downy mildew, caused byPlasmopara viticola, is commonly controlled by quinone outside inhibitor (QoI) and carboxylic acid amide (CAA) fungicides. QoI resistance is conferred by a mutation resulting in a G143A alteration in cytochromeb, while CAA resistance is associated with a G1105S mutation in cellulose synthase 3. The aims of this study were to (a) verify the presence of QoI and CAA resistance inP.viticolain Brazil, (b) develop a single nucleotide polymorphism (SNP) detection pyrosequencing assay for quantitative detection of G143A, and (c) determine the geographic prevalence and distribution of G143A and G1105S in Brazilian field populations.P.viticolaisolates were sampled from two vineyards reporting failures of QoI and CAA control. Their growth was unaffected by azoxystrobin and mandipropamid, and resistance was associated with the G143A and G1105S mutations, respectively. The SNP detection pyrosequencing assay developed for G143A detection was precise and accurate. Pyrosequencing assays revealed that G143A and G1105S frequencies varied among the 66 field populations and were positively correlated with the number of QoI and CAA applications, respectively. The G143A mutation was observed in 60 populations, 39 of them with frequencies over 90%. G1105S was detected in 20 populations with frequencies ranging from 18.3% to 100%, and which also carried the G143A mutation. Multiple resistance to QoI and CAA inP.viticolawas confirmed for the first time in South America. Future resistance monitoring surveys and management strategies must be adopted to prevent further increase in QoI and CAA resistance in Brazil. (AU)

• Processo FAPESP: 18/25596-7 - Caracterização molecular de Plasmopara viticola e Phakopsora meliosmae-myrianthae com diferentes níveis de sensibilidade a fungicidas QoI e DMI no Brasil
• Beneficiário: Ricardo Feliciano dos Santos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 17/21412-6 - Estrutura populacional e sensibilidade de Plasmopara viticola e Phakopsora euvitis a fungicidas no Brasil
• Beneficiário: Ricardo Feliciano dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/24003-9 - Epidemiologia, avaliação de danos e controle de doenças da videira
• Beneficiário: Lilian Amorim
• Modalidade de apoio: Auxílio à Pesquisa - Temático