Perinatal fluoxetine treatment promotes long-term behavioral changes in adult mice

Autor(es)

Laureano-Melo, Roberto ^{[1, 2]} ; Dos-Santos, Raoni Conceicao ^[1] ; da Conceicao, Rodrigo Rodrigues ^[3] ; de Souza, Janaina Sena ^[3] ; Lau, Raphael da Silva ^[1] ; Souza Silva, Samantha da Silva ^[1] ; Marinho, Bruno Guimaraes ^[1] ; Giannocco, Gisele ^[3] ; Ahmed, R. G. ^[4] ; Cortes, Wellington da Silva ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Rural Rio de Janeiro, Multictr Grad Program Physiol Sci, Inst Hlth & Biol Sci, Dept Physiol Sci, Seropedica - Brazil

^[2] Barra Mansa Univ Ctr, Dept Vet Med, Rio De Janeiro - Brazil

^[3] Univ Fed Sao Paulo, Dept Med, Mol & Translat Endocrinol Lab, Sao Paulo, SP - Brazil

^[4] Beni Suef Univ, Fac Sci, Zool Dept, Div Anat & Embryol, Bani Suwayf - Egypt

• Tipo de documento: Artigo Científico
• Fonte: METABOLIC BRAIN DISEASE; v. 35, n. 8 AUG 2020.
• Citações Web of Science: 0

Resumo

Serotonin exerts a significant role in the mammalian central nervous system embryogenesis and brain ontogeny. Therefore, we investigate the effect of perinatal fluoxetine (FLX), a selective serotonin reuptake inhibitor, administration on the behavioral expression of adult male Swiss mice. For this purpose, two groups (n = 6 each, and similar to 35 g) of pregnant female Swiss mice were mated. Their offspring were treated with FLX (10 mg/Kg, s.c.) from postnatal day (PND) 5 to 15. At PND 16, one male puppy of each litter was euthanized, and the hippocampus was dissected for RNA analysis. At 70 days of life, the male offspring underwent a behavioral assessment in the open field, object recognition task, light-dark box, tail suspension and rotarod test. According to our results, the programmed animals had a decrease in TPH2, 5HT1a, SERT, BDNF, and LMX1B expression. Also, it was observed less time of immobility in tail suspension test and higher grooming time in the open field test. In the light-dark box test, the FLX-treated offspring had less time in the light side than control. We also observed a low cognitive performance in the object recognition task and poor motor skill learning in the rotarod test. These findings suggest that programming with FLX during the neonatal period alters a hippocampal serotonergic system, promoting anxiety and antidepressant behavior in adults, as well as a low mnemonic capacity. (AU)

• Processo FAPESP: 18/22763-0 - DNA antigo, bioengenharia e "organoides" de cérebro: estudo da homeostase do hormônio tiroidiano e marcadores da Doença de Alzheimer em células tronco com gene NOVA1 editado por CRISPR
• Beneficiário: Janaína Sena de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 17/07053-3 - Efeito do hormônio tiroidiano no encéfalo de camundongos 3xTg-AD, modelo da Doença de Alzheimer, no metabolismo da glicose e colesterol
• Beneficiário: Janaína Sena de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/23169-1 - Efeito dos hormônios tiroidianos no encéfalo de camundongos 3xTg-AD (APPswe, PS1m146v, tauP301L) modelo da doença de Alzheimer
• Beneficiário: Gisele Giannocco
• Modalidade de apoio: Auxílio à Pesquisa - Regular