Radiotherapy Activates Matrix Metalloproteinases in the Dentinoenamel Junction of Primary Teeth

Autor(es)

Queiroz, Alexandra M. ^[1] ; Carpio-Bonilla, Claudia M. ^[1] ; Arnez, Maya F. M. ^[1] ; dos Santos, Thais T. ^[1] ; Palma-Dibb, Regina G. ^[2] ; Oliveira, Harley F. ^{[3, 4]} ; Nelson-Filho, Paulo ^[1] ; Silva, Lea A. B. ^[1] ; Paula-Silva, Francisco W. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Restorat Dent, Ribeirao Preto, SP - Brazil

^[3] Marcio Cunha Hosp, Ipatinga, MG - Brazil

^[4] Radiooncol Treatment Ctr, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DENTISTRY FOR CHILDREN; v. 87, n. 2, p. 83-89, MAY 2020.
• Citações Web of Science: 0

Resumo

Purpose: Radiation-related caries is characterized by enamel delamination near the dentinoenamel junction (DEJ). We investigated the activity and expression of the matrix metalloproteinases (MMPs) -2 and -9 in order to understand disease pathogenesis in teeth submitted or not to radiotherapy (RT). Methods: In situ zymography and immunofluorescence assays were performed to evaluate the activity and expression of MMPs -2 and -9, respectively. Twelve primary second molars were randomly assigned into two experimental subgroups: irradiated and nonirradiated. Dental fragments were exposed to radiation at a dose fraction of two Gy for five consecutive days until reaching the total dose of 60 Gy. The per-centage of fluorescence in the DEJ was evaluated in three distinct regions of the tooth (cervical, cusp, and pit). The regions were photographed under fluorescence microscopy at 1.25x and 5x magnification. Results: The intensity of fluorescence per mm(2) in the DEJ was higher in the cervical region of irradiated primary teeth (P<0.05) versus nonirradiated ones. In these areas, immunofluorescence revealed expression of MMPs -2 and -9. Conclusion: Radiotherapy can increase the activity of MMPs -2 and -9 in the cervical region of the DEJ of primary teeth. (AU)

• Processo FAPESP: 16/16243-8 - Influência da radioterapia na alteração de cor do binômio esmalte dental / restaurações de resina composta
• Beneficiário: Thais Tedeschi dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/17611-4 - Mecanismos envolvidos na modulação da via 5-lipoxigenase na lesão periapical experimentalmente induzida
• Beneficiário: Francisco Wanderley Garcia de Paula e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores