“Half-Sandwich”/RuII Anticancer Complexes Containing Triphenylphosphine and p-Substituted Benzoic Acids

Autor(es)

João Honorato ^[1] ; Katia M. Oliveira ^[2] ; Celisnolia M. Leite ^[3] ; Legna Colina-Vegas ; Joaquim A. Nóbrega ^[5] ; Eduardo E. Castellano ^[6] ; Javier Ellena ^[7] ; Rodrigo S. Correa ^[8] ; Alzir A. Batista

Afiliação do(s) autor(es)

^[1] Universidade Federal de São Carlos (UFSCar). Departamento de Química - Brasil

^[2] Universidade Federal de Ouro Preto (UFOP). Instituto de Ciências Exatas e Biológicas (ICEB). Departamento de Química - Brasil

^[3] Universidade Federal de São Carlos (UFSCar). Departamento de Química - Brasil

^[5] Universidade Federal de São Carlos (UFSCar). Departamento de Química - Brasil

^[6] Universidade de São Paulo (USP). Instituto de Física de São Carlos - Brasil

^[7] Universidade de São Paulo (USP). Instituto de Física de São Carlos - Brasil

^[8] Universidade Federal de Ouro Preto (UFOP). Instituto de Ciências Exatas e Biológicas (ICEB). Departamento de Química - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 31, n. 11, p. 2237-2249, 2020-10-30.

Resumo

Mononuclear and binuclear RuII/arene/triphenylphosphine complexes with p-substituted benzoic acid derivatives were prepared and characterized. These monocationic complexes of type [Ru(η6-p-cymene)(PPh3)L] (L = benzoic acid (1), p-hydroxybenzoic acid (2), p-nitrobenzoic acid (3) and terephthalic acid (4)) were characterized using various techniques, such as nuclear magnetic resonance (NMR) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, and the crystal structure of 1, 3 and 4 were determined by X-ray diffraction analysis. The cytotoxicity of the complexes was evaluated, in vitro, against tumorigenic [MDA-MB-231, MCF-7 (breast), A549 (lung) and DU-145 (prostate)] and non-tumorigenic [MCF-10A (breast), MRC-5 (lung) and PNT-2 (prostate)] cells. The binuclear complex (4) was inactive due to its low solubility. Complexes 1, 2 and 3 showed similar cytotoxicity, however, complex 1 presented better selectivity index against MDA-MB-231 than compounds 2 and 3. Cellular ruthenium absorption was explored by inductively coupled plasma mass spectrometry (ICP-MS) analyzing the whole cells and the culture medium. Complementary studies showed that complex 1 inhibited colony formation, induced morphology changes in cells and promoted cell cycle arrest in the Sub-G1 phase for the MDA-MB-231 cells. (AU)

• Processo FAPESP: 16/23130-5 - Determinação da biodistribuição celular de Cu, Ru e Pt em células humanas tumorais e não tumorais de mama mediante a técnica de espectrometria de massas com plasma acoplado indutivamente
• Beneficiário: Legna Andreina Colina Vegas
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado