Isolation of intact Leishmania amazonensis large parasitophorous vacuoles from infected macrophages by density gradient fractionation

Autor(es)

Real, Fernando

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 218, NOV 2020.
• Citações Web of Science: 0

Resumo

As the causative agent of hard-to-treat diffuse cutaneous leishmaniasis, Leishmania (L.) amazonensis persists in the host organism sheltered within large Parasitophorous Vacuoles (PVs) formed mainly in macrophages. In the present study, I present a simple and efficient method for L. amazonensis PV isolation. Isolated PVs are intact as demonstrated by the conservation of lysosomal probes loaded into PVs before the procedure. The method is useful for studies aiming at a complete and accurate molecular profile of these structures, to better understand the biogenesis of this pathogen-containing vacuole and its implication in parasite persistence and in leishmaniasis pathogenesis. (AU)

• Processo FAPESP: 14/25296-2 - Identificação dos requisitos mínimos para a sobrevivência de Leishmania em células hospedeiras: RNA-seq de fibroblastos L929 enucleados infectados por Leishmania amazonensis
• Beneficiário: Renato Arruda Mortara
• Modalidade de apoio: Auxílio à Pesquisa - Regular