| Texto completo | |
| Autor(es): |
Sousa, Maria E. P.
[1]
;
Gonzatti, Michelangelo B.
[1]
;
Fernandes, Edgar R.
[1]
;
Freire, Beatriz M.
[1]
;
Guereschi, Marcia G.
[1]
;
Basso, Alexandre S.
[1]
;
Andersen, Monica L.
[2]
;
Rosa, Daniela S.
[3, 1]
;
Keller, Alexandre C.
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Fed Univ Sao Paulo UNIFESP EPM, Dept Microbiol Immunol & Parasitol, Rua Botucatu, 4th Floor, BR-04023062 Sao Paulo - Brazil
[2] Fed Univ Sao Paulo UNIFESP EPM, Dept Psychobiol, Rua Botucatu, 862, 1st Floor, BR-04023062 Sao Paulo - Brazil
[3] Inst Invest Immunol Iii INCT, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BRAIN BEHAVIOR AND IMMUNITY; v. 90, p. 208-215, NOV 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Although several studies demonstrate that stressful situations, such as sleep disturbances, negatively impact the innate and adaptive arms of the immune system, their influence on invariant Natural Killer T (iNKT) cells remains unclear. iNKT cells are CD1d-restricted innate T cells that recognize glycolipid antigens and rapidly produce polarizing cytokines being key players in several immune responses, and a potential target for immunotherapy. iNKT cells differ in several aspects from conventional T lymphocytes, including a unique dependence on CD1d-expressing double-positive (DP) thymocytes for intrathymic maturation. As a consequence of stress, DP thymocytes undergo glucocorticoid-induced apoptosis, which might compromise iNKT developmental pathway. Therefore, we used a paradoxical sleep deprivation (SD) model to determine the impact of sleep disturbance on iNKT cell biology. After 72 h of SD, C57Bl/6 mice exhibited a significant increase in systemic glucocorticoid levels and thymus atrophy. Despite marked decrease in the number of DP thymocytes, the ratio CD1d(+)/CD1d(-) was higher in SD mice, and the number of thymic iNKT cells remained unaltered, suggesting that SD did not compromise the iNKT developmental pathway. In contrast, SD reduced hepatic IFN-gamma, but not, IL-4-producing iNKT cells, without further effect in the spleen. Despite this fact, SD did not affect stimulation of IFN-gamma production by iNKT cells, or cytokine release, in response to alpha-galactosylceramide, a specific antigen. Furthermore, although SD impaired splenic NK cells activity against tumor cells, it did not affect iNKT cell-specific cytotoxicity. Thus, our study shows that SD-induced stress did not impair the iNKT cells' responses to a cognate antigen. (AU) | |
| Processo FAPESP: | 17/17471-7 - Antigenicidade e imunogenicidade de proteínas recombinantes do envelope viral do Zika vírus |
| Beneficiário: | Daniela Santoro Rosa |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/11490-5 - Estudo sobre a influência do sistema nervoso simpático na atividade biológica dos linfócitos T invariantes natural killer |
| Beneficiário: | Alexandre de Castro Keller |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |