The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin

Autor(es)

de Farias, Aline Leite ^[1] ; Meneguin, Andreia Bagliotti ^[2] ; Barud, Hernane da Silva ^[3] ; Brighenti, Fernanda Lourencao ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Morphol Pediat Dent & Orthodont, BR-14801903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Dept Drugs & Pharmaceut, BR-14801903 Araraquara, SP - Brazil

^[3] Univ Araraquara UNIARA, Biopolymers & Biomat Lab BioPolMat, R Carlos Gomes 1217, BR-14801340 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 162, p. 1944-1958, NOV 1 2020.
• Citações Web of Science: 0

Resumo

The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity. Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin. The systems were characterized in terms of morphological characteristics, mucoadhesion and in vitro drug release. Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms. Scanning Electron Microscopy demonstrated that the microparticles were spherical, rough and compact. The film and the tablet presented smooth and continuous surface and in the inner of the tablet was porous. These systems were more mucoadhesive compared to the microparticles. The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing. The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group. The findings of this study showed that the morin has antibiofilm activity against cariogenic microorganisms. (C) 2020 Published by Elsevier B.V. (AU)

• Processo FAPESP: 13/12326-8 - Interação microbiana entre bactérias cariogênicas: avaliação do crescimento, da atividade metabólica, da composição bioquímica e da suscetibilidade à clorexidina de biofilmes formados in vitro
• Beneficiário: Fernanda Lourenção Brighenti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 18/25512-8 - Plataforma para Cultura de Células Baseada em Biocelulose
• Beneficiário: Hernane da Silva Barud
• Modalidade de apoio: Auxílio à Pesquisa - Regular