A Simple and Fast Battery Test for Phenotypic Characterization of Mice

Autor(es):Mostrar menos -

Garcia-Gomes, Mariana S. A. ^[1] ; Zanatto, Dennis A. ^[1] ; Yamamoto, Pedro K. ^[1] ; Wadt, Danilo ^[1] ; Antiorio, Ana T. F. B. ^[1] ; Aleman-Laporte, Jilma ^[1] ; Alexandre-Ribeiro, Sandra R. ^[2] ; Marson, Guilherme A. ^[3] ; Guizzo, Cezar ^[3] ; Massironi, Silvia M. G. ^[1] ; Bernardi, Maria M. ^[4] ; Mori, Claudia M. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Chem, Chem Dept, Sao Paulo - Brazil

^[4] Univ Paulista, Grad Program Environm & Expt Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIO-PROTOCOL; v. 10, n. 7 APR 5 2020.
• Citações Web of Science: 0

Resumo

Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or genetically modified mice. This methodology is simple and efficient in order to analyze several parameters, including general activity, sensory nervous system, sensorimotor system, central nervous system and autonomous nervous system. This can aid the choice of specific additional tests as well as the determination of an interrelationship among phenotypic alterations observed. Although being efficient for a first analysis of a mouse model, interpretation of the results must be carefully made because phenotype manifestation may vary due to many parameters, including mouse strain, environmental and housing condition, animal-experimenter interaction, sample size and tests order. It is important to consider as a critical point if handling procedures are aversive. The results acquired with the analysis of 18 parameters together provide preliminary data to characterize mouse phenotype and helps selecting more specific tests. (AU)

• Processo FAPESP: 17/21103-3 - Caracterização fenotípica e molecular de duas linhagens de camundongos mutantes que apresentam alterações motoras e sensoriais como potenciais modelos para estudo de doenças neurológicas
• Beneficiário: Claudia Madalena Cabrera Mori
• Modalidade de apoio: Auxílio à Pesquisa - Regular