In vivo Activity of Silver Nanoparticles Against Pseudomonas aeruginosa Infection in Galleria mellonella

Autor(es)

Thomaz, Luciana ^[1] ; de Almeida, Luiz Gustavo ^[1] ; Silva, Flavia R. O. ^[2] ; Cortez, Mauro ^[3] ; Taborda, Carlos P. ^{[1, 4]} ; Spira, Beny ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[2] Nucl & Energy Res Inst IPEN, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Trop Med Sao Paulo, Fac Med, Lab Med Mycol LIM53, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 11, NOV 9 2020.
• Citações Web of Science: 0

Resumo

Pseudomonas aeruginosa is an opportunistic pathogen associated with life-threatening nosocomial and community-acquired infections. Antibiotic resistance is an immediate threat to public health and demands an urgent action to discovering new antimicrobial agents. One of the best alternatives for pre-clinical tests with animal models is the greater wax moth Galleria mellonella. Here, we evaluated the antipseudomonal activity of silver nanoparticles (AgNPs) against P. aeruginosa strain UCBPP-PA14 using G. mellonella larvae. The AgNPs were synthesized through a non-toxic biogenic process involving microorganism fermentation. The effect of AgNPs was assessed through characterization and quantification of the hemocytic response, nodulation and phenoloxidase cascade. On average, 80% of the larvae infected with P. aeruginosa and prophylactically treated with nanoparticles survived. Both the specific and total larvae hemocyte counts were restored in the treated group. In addition, the nodulation process and the phenoloxidase cascade were less exacerbated when the larvae were exposed to the silver nanoparticles. AgNPs protect the larvae from P. aeruginosa infection by directly killing the bacteria and indirectly by preventing an exacerbated immunological response against the pathogen. Our results suggest that the prophylactic use of AgNPs has a strong protective activity against P. aeruginosa infection. (AU)

• Processo FAPESP: 12/24105-3 - Imunobiologia de Leishmania spp.: estudo do papel de CD200 e biogênese do vacúolo parasitóforo em células fagocíticas infectadas por Leishmania
• Beneficiário: Mauro Javier Cortez Véliz
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/06547-7 - Desenvolvimento de modelos alternativos, na paracoccidioidomicose experimental, para avaliação da patogenicidade e tratamento com anticorpos monoclonais em Galleria mellonella
• Beneficiário: Luciana Thomaz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/08730-6 - Patogenicidade fúngica: efeito do tabagismo, resposta imune e a modulação vacinal na paracoccidioidomicose e na histoplasmose
• Beneficiário: Carlos Pelleschi Taborda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/07775-2 - Acúmulo de polifosfato e o papel do gene phoU em Pseudomonas aeruginosa
• Beneficiário: Beny Spira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/09143-1 - O regulon PHO e evolução dirigida em Pseudomonas aeruginosa
• Beneficiário: Luiz Gustavo de Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado