| Full text | |
| Author(s): |
Thomaz, Luciana
[1]
;
de Almeida, Luiz Gustavo
[1]
;
Silva, Flavia R. O.
[2]
;
Cortez, Mauro
[3]
;
Taborda, Carlos P.
[1, 4]
;
Spira, Beny
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[2] Nucl & Energy Res Inst IPEN, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Trop Med Sao Paulo, Fac Med, Lab Med Mycol LIM53, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FRONTIERS IN MICROBIOLOGY; v. 11, NOV 9 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Pseudomonas aeruginosa is an opportunistic pathogen associated with life-threatening nosocomial and community-acquired infections. Antibiotic resistance is an immediate threat to public health and demands an urgent action to discovering new antimicrobial agents. One of the best alternatives for pre-clinical tests with animal models is the greater wax moth Galleria mellonella. Here, we evaluated the antipseudomonal activity of silver nanoparticles (AgNPs) against P. aeruginosa strain UCBPP-PA14 using G. mellonella larvae. The AgNPs were synthesized through a non-toxic biogenic process involving microorganism fermentation. The effect of AgNPs was assessed through characterization and quantification of the hemocytic response, nodulation and phenoloxidase cascade. On average, 80% of the larvae infected with P. aeruginosa and prophylactically treated with nanoparticles survived. Both the specific and total larvae hemocyte counts were restored in the treated group. In addition, the nodulation process and the phenoloxidase cascade were less exacerbated when the larvae were exposed to the silver nanoparticles. AgNPs protect the larvae from P. aeruginosa infection by directly killing the bacteria and indirectly by preventing an exacerbated immunological response against the pathogen. Our results suggest that the prophylactic use of AgNPs has a strong protective activity against P. aeruginosa infection. (AU) | |
| FAPESP's process: | 12/24105-3 - Immunobiology of Leishmania spp.: study of the role of CD200 and biogenesis of parasitophorous vacuole in fagocitic cells infected by Leishmania |
| Grantee: | Mauro Javier Cortez Véliz |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/06547-7 - Development of alternative models in the experimental paracoccidioidomycosis to evaluate the pathogenicity and treatment with monoclonal antibodies in Galleria mellonella |
| Grantee: | Luciana Thomaz |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/08730-6 - Fungal pathogenicity: environmental effects, immune response and vaccine modulation in the Brazilian endemic mycoses paracoccidioidomycosis and histoplasmosis |
| Grantee: | Carlos Pelleschi Taborda |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 11/07775-2 - Polyphosphate accumulation and the role of phoU in Pseudomonas aeruginosa |
| Grantee: | Beny Spira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/09143-1 - The PHO regulon and assisted evolution in Pseudomonas aeruginosa |
| Grantee: | Luiz Gustavo de Almeida |
| Support Opportunities: | Scholarships in Brazil - Doctorate |