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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In Situ Proteomic Analysis of Glioblastoma Multiforme: A Translational Approach to Improve Prognostic/Diagnostic Routines

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Autor(es):
da Silva Menegasso, AnaIly Ribeiro [1] ; Pratavieira, Marcel [1] ; dos Santos, Lucilene Delazari [2] ; Lima, Flavio de Oliveira [2] ; Moraes, Marcelo Padovani T. [3] ; Zanini, Marco Antonio [3] ; Palma, Mario Sergio [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Ctr Study Social Insects, Dept Basic & Appl Biol, Inst Biosci, Rio Claro, SP - Brazil
[2] Sao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Botucatu Med Sch FMB, Botucatu, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY; v. 7, n. 29, p. 64-83, OCT-DEC 2020.
Citações Web of Science: 0
Resumo

In order to evaluate the use of matrix-assisted laser desorption ionization (MALDI) Mass Spectral Imaging (MSI) to Glioblastoma (GBM) studies, some sections of formalin fixed paraffin embedded samples of GBM tumors were submitted to classical immunoassays, to define the profile of distribution of some classical and well recognized molecular markers of GBM grade IV (Ki-67, S100, Glial GFAP, CD31 and CD34), while other sections of the same samples were submitted to in-tissueproteomic analysis by MALDI MSI, and both results compared to each other. The overlapping of the MALDI spectra obtained for the tryptic peptides with the immunohistochemical reactions of each marker protein were used to build a distribution map of the marker proteins all over the GBM tissue section. The results revealed a high correlation between both methods, indicating that MALDI MSI has enough sensitivity to be compared to the immunohistochemical methods, as well is sufficiently reliable to be used in biomarkers identification. (AU)

Processo FAPESP: 16/16212-5 - Proteopeptídeos naturais da fauna, flora e microbiota brasileira como potenciais modelos para o desenvolvimento racional de novos fármacos de uso terapêutico: isolamento, elucidação estrutural, síntese química e ensaios de atividade funcional
Beneficiário:Mario Sergio Palma
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático