Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The role of glucose metabolism and insulin resistance in cardiac remodelling induced by cigarette smoke exposure

Texto completo
Autor(es):
Mostrar menos -
Azevedo, Paula Schmidt [1] ; Polegato, Bertha F. [1] ; Paiva, Sergio [1] ; Costa, Nara [2] ; Santos, Priscila [1] ; Bazan, Silmeia [1] ; Henrique Fernandes, Ana Angelica [3] ; Fabro, Alexandre [4] ; Pires, Vanessa [1] ; Tanni, Suzana E. [1] ; Pereira, Filipe Leal [1] ; Lo, Angelo [1] ; Grassi, Leticia [1] ; Campos, Dijon [4] ; Androcioli, Vickeline [5] ; Zornoff, Leonardo [1] ; Minicucci, Marcos [1]
Número total de Autores: 17
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Internal Med, Av Prof Mario Rubens Guimaraes Montenegro S-N, BR-18618687 Botucatu, SP - Brazil
[2] UFG Univ Fed Goias, Fac Nutr, Goiania, Go - Brazil
[3] Sao Paulo State Univ UNESP, Dept Chem & Biol Sci, Botucatu, SP - Brazil
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol & Legal Med, Ribeirao Preto - Brazil
[5] Sao Paulo State Univ UNESP, Botucatu Med Sch, Expt Res Unit UNIPEX, Botucatu, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE; v. 25, n. 2 DEC 2020.
Citações Web of Science: 0
Resumo

The aim of this study is to evaluate whether the alterations in glucose metabolism and insulin resistance are mechanisms presented in cardiac remodelling induced by the toxicity of cigarette smoke. Male Wistar rats were assigned to the control group (C; n = 12) and the cigarette smoke-exposed group (exposed to cigarette smoke over 2 months) (CS; n = 12). Transthoracic echocardiography, blood pressure assessment, serum biochemical analyses for catecholamines and cotinine, energy metabolism enzymes activities assay; HOMA index (homeostatic model assessment); immunohistochemistry; and Western blot for proteins involved in energy metabolism were performed. The CS group presented concentric hypertrophy, systolic and diastolic dysfunction, and higher oxidative stress. It was observed changes in energy metabolism, characterized by a higher HOMA index, lower concentration of GLUT4 (glucose transporter 4) and lower 3-hydroxyl-CoA dehydrogenase activity, suggesting the presence of insulin resistance. Yet, the cardiac glycogen was depleted, phosphofructokinase (PFK) and lactate dehydrogenase (LDH) increased, with normal pyruvate dehydrogenase (PDH) activity. The activity of citrate synthase, mitochondrial complexes and ATP synthase (adenosine triphosphate synthase) decreased and the expression of Sirtuin 1 (SIRT1) increased. In conclusion, exposure to cigarette smoke induces cardiac remodelling and dysfunction. The mitochondrial dysfunction and heart damage induced by cigarette smoke exposure are associated with insulin resistance and glucose metabolism changes. (AU)

Processo FAPESP: 14/24197-0 - O papel do metabolismo da glicose na cardiomiopatia induzida pela exposição à fumaça de cigarros
Beneficiário:Paula Schmidt Azevedo Gaiolla
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 16/14547-0 - Influência da metformina no metabolismo energético cardíaco de ratos expostos á fumaça de cigarros
Beneficiário:Vanessa de Cássia Martins Pires Ferreira
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 16/25676-5 - Influência da metformina no remodelamento cardíaco de ratos expostos à fumaça de cigarros
Beneficiário:Letícia Dalla Vecchia Grassi
Linha de fomento: Bolsas no Brasil - Iniciação Científica