Yeasts from indigenous culture for cachaca production and brewer's spent grain: Biodiversity and phenotypic characterization for biotechnological purposes

Autor(es)

Brexo, Ramon P. ^[1] ; Brandao, Luciana R. ^[2] ; Chaves, Rafael D. ^[1] ; Castro, Ruann J. S. ^[1] ; Camara, Jr., Antonio A. ^[1] ; Rosa, Carlos A. ^[2] ; Sant'Ana, Anderson S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, Campinas - Brazil

^[2] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FOOD AND BIOPRODUCTS PROCESSING; v. 124, p. 107-120, NOV 2020.
• Citações Web of Science: 0

Resumo

In this study, yeasts were isolated from an indigenous starter culture for cachac a production (Brazilian spirit) and brewer's spent grain and characterized through a series of phenotypic assays: the killer profile, ascospore formation capacity, growth at high temperatures, resistance to natamycin and actidione, and exoenzyme production. One hundred thirty-four (n = 134) yeasts were isolated and identified as belonging to 6 genera and 10 different species. The ascospore formation in potassium acetate agar was observed in 86% of the isolates (73 Saccharomyces cerevisiae, 3 Torulaspora delbrueckii, and 1 Wickerhamomyces anomalus) highlighting their potential for microencapsulation. Only S. cerevisiae LMQA SNR 70 expressed an effective killer factor (ability to destroy sensitive strains), while only 3 S. cerevisiae, 1 Clavispora lusitaniae, and 1 Pichia kudriavzevii isolates were able to grow up at 45 ?C. At 50 and 100 mg/L, actidione showed selectivity action for Candida parapsilosis, while natamycin inhibited the growth of all isolates. The non-Saccharomyces isolates stood out in the qualitative and quantitative tests for amylolytic, pectinolytic, cellulolytic, and xylanolytic activities. A predominance of the species W. anomalus and T. delbrueckii was observed. Besides, quantitative tests revealed that 23% of the isolates exhibited exoenzymatic activity in a nutrient-poor environment, and W. anomalus LMQA CSC 5 e LMQA CSC 43 stood out for the multi-enzymatic in agar and liquid medium response. The phenotypic profile showed that 16% of the isolates expressed two or more characteristics studied, with 2/3 represented by the non-Saccharomyces species. These results highlight the importance of studying yeast species for new biotechnological purposes. (C) 2020 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 18/19311-0 - Produção e estabilização de novas leveduras starter isoladas de queijos artesanais brasileiros
• Beneficiário: Antonio de Anchieta Câmara Júnior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado