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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Yeasts from indigenous culture for cachaca production and brewer's spent grain: Biodiversity and phenotypic characterization for biotechnological purposes

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Author(s):
Brexo, Ramon P. [1] ; Brandao, Luciana R. [2] ; Chaves, Rafael D. [1] ; Castro, Ruann J. S. [1] ; Camara, Jr., Antonio A. [1] ; Rosa, Carlos A. [2] ; Sant'Ana, Anderson S. [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, Campinas - Brazil
[2] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Belo Horizonte, MG - Brazil
Total Affiliations: 2
Document type: Journal article
Source: FOOD AND BIOPRODUCTS PROCESSING; v. 124, p. 107-120, NOV 2020.
Web of Science Citations: 0
Abstract

In this study, yeasts were isolated from an indigenous starter culture for cachac a production (Brazilian spirit) and brewer's spent grain and characterized through a series of phenotypic assays: the killer profile, ascospore formation capacity, growth at high temperatures, resistance to natamycin and actidione, and exoenzyme production. One hundred thirty-four (n = 134) yeasts were isolated and identified as belonging to 6 genera and 10 different species. The ascospore formation in potassium acetate agar was observed in 86% of the isolates (73 Saccharomyces cerevisiae, 3 Torulaspora delbrueckii, and 1 Wickerhamomyces anomalus) highlighting their potential for microencapsulation. Only S. cerevisiae LMQA SNR 70 expressed an effective killer factor (ability to destroy sensitive strains), while only 3 S. cerevisiae, 1 Clavispora lusitaniae, and 1 Pichia kudriavzevii isolates were able to grow up at 45 ?C. At 50 and 100 mg/L, actidione showed selectivity action for Candida parapsilosis, while natamycin inhibited the growth of all isolates. The non-Saccharomyces isolates stood out in the qualitative and quantitative tests for amylolytic, pectinolytic, cellulolytic, and xylanolytic activities. A predominance of the species W. anomalus and T. delbrueckii was observed. Besides, quantitative tests revealed that 23% of the isolates exhibited exoenzymatic activity in a nutrient-poor environment, and W. anomalus LMQA CSC 5 e LMQA CSC 43 stood out for the multi-enzymatic in agar and liquid medium response. The phenotypic profile showed that 16% of the isolates expressed two or more characteristics studied, with 2/3 represented by the non-Saccharomyces species. These results highlight the importance of studying yeast species for new biotechnological purposes. (C) 2020 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 18/19311-0 - Production and stabilization of new starter yeasts isolated from Brazilian artisanal cheeses
Grantee:Antonio de Anchieta Câmara Júnior
Support Opportunities: Scholarships in Brazil - Post-Doctoral