Biseugenol Exhibited Anti-Inflammatory and Anti-Asthmatic Effects in an Asthma Mouse Model of Mixed-Granulocytic Asthma

Autor(es):Mostrar menos -

Ponci, Vitor ^[1] ; Silva, Rafael C. ^[2] ; Santana, Fernanda Paula R. ^[2] ; Grecco, Simone S. ^[3] ; Fortunato, Celia Regina M. ^[3] ; Oliveira, Maria A. ^[4] ; Tavares-de-Lima, Wothan ^[4] ; Olivo, Clarice R. ^[5] ; Calvo Tiberio, Iolanda de Fatima L. ^[5] ; Gomes, Kaio S. ^[6] ; Prado, Carla M. ^[7] ; Lago, Joao Henrique G. ^[6]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Chem, BR-09972270 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biol Sci, BR-09972270 Sao Paulo - Brazil

^[3] Univ Anhanguera, Dept Biotechnol & Hlth Innovat, BR-05145200 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508000 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Med, BR-01246000 Sao Paulo - Brazil

^[6] Univ Fed ABC, Human & Nat Sci Ctr, BR-09210580 Sao Paulo - Brazil

^[7] Univ Fed Sao Paulo, Dept Biosci, BR-11015020 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 25, n. 22 NOV 2020.
• Citações Web of Science: 0

Resumo

In the present work, the anti-inflammatory and antiasthmatic potential of biseugenol, isolated as the main component from n-hexane extract from leaves of Nectandra leucantha and chemically prepared using oxidative coupling from eugenol, was evaluated in an experimental model of mixed-granulocytic asthma. Initially, in silico studies of biseugenol showed good predictions for drug-likeness, with adherence to Lipinski's rules of five (RO5), good Absorption, Distribution, Metabolism and Excretion (ADME) properties and no alerts for Pan-Assay Interference Compounds (PAINS), indicating adequate adherence to perform in vivo assays. Biseugenol (20 mg center dot kg(-1)) was thus administered intraperitoneally (four days of treatment) and resulted in a significant reduction in both eosinophils and neutrophils of bronchoalveolar lavage fluid in ovalbumin-sensitized mice with no statistical difference from dexamethasone (5 mg center dot kg(-1)). As for lung function parameters, biseugenol (20 mg center dot kg(-1)) significantly reduced airway and tissue damping in comparison to ovalbumin group, with similar efficacy to positive control dexamethasone. Airway hyperresponsiveness to intravenous methacholine was reduced with biseugenol but was inferior to dexamethasone in higher doses. In conclusion, biseugenol displayed antiasthmatic effects, as observed through the reduction of inflammation and airway hyperresponsiveness, with similar effects to dexamethasone, on mixed-granulocytic ovalbumin-sensitized mice. (AU)

• Processo FAPESP: 18/06088-0 - Efeito do tratamento com eugenol e deidrodieugenol isolados de Nectandra leucantha (Lauraceae) em modelos experimentais de doenças pulmonares agudas e crônicas.
• Beneficiário: Carla Máximo Prado
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/07885-1 - Biomoléculas oriundas de espécies vegetais de áreas remanescentes da Mata Atlântica e do Cerrado para tratamento de doenças tropicais negligenciadas - aspectos químicos e farmacológicos
• Beneficiário: João Henrique Ghilardi Lago
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular