Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1 beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis

Autor(es)

Carriero, Mateus Maldonado ^[1] ; Henrique-Silva, Flavio ^[2] ; Meira, Caroline Munhoz ^[1] ; Queiroz Gato, Igor Mateus ^[1] ; Caetano, Alexandre Rodrigues ^[3] ; Lobo, Francisco Pereira ^[4] ; Alves, Anderson Luis ^[5] ; Varela, Eduardo Sousa ^[5] ; Mendes Maia, Antonio Augusto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil

^[3] Empresa Brasileira Pesquisa Agr, Embrapa Recursos Genet & Biotecnol, Brasilia, DF - Brazil

^[4] Empresa Brasileira Pesquisa Agr, Embrapa Informat Agr, Campinas, SP - Brazil

^[5] Empresa Brasileira Pesquisa Agr, Embrapa Pesca & Aquicultura, Palmas, TO - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 43, n. 4 2020.
• Citações Web of Science: 0

Resumo

In the present study, the complete characterization of cDNA and genomic sequences of IL-1 beta and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1 beta cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1 beta, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive m RNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1 beta and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pm IL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups. (AU)

• Processo FAPESP: 17/26996-6 - Avaliação da triploidia na susceptibilidade à infecção por Aeromonas jandaei e possível relação com os níveis de expressão de genes de resposta imune em lambaris (Astyanax altiparanae)
• Beneficiário: Antonio Augusto Mendes Maia
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/18562-6 - Imunização de lambaris (Astyanax altiparanae) com vacina gênica contendo o antígeno de imobilização IAG52A de Ichthyophthirius multifiliis em conjunto com a citocina IL-8 de A. altiparanae como adjuvante molecular
• Beneficiário: Mateus Maldonado Carriero
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado