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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1 beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis

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Author(s):
Carriero, Mateus Maldonado [1] ; Henrique-Silva, Flavio [2] ; Meira, Caroline Munhoz [1] ; Queiroz Gato, Igor Mateus [1] ; Caetano, Alexandre Rodrigues [3] ; Lobo, Francisco Pereira [4] ; Alves, Anderson Luis [5] ; Varela, Eduardo Sousa [5] ; Mendes Maia, Antonio Augusto [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[3] Empresa Brasileira Pesquisa Agr, Embrapa Recursos Genet & Biotecnol, Brasilia, DF - Brazil
[4] Empresa Brasileira Pesquisa Agr, Embrapa Informat Agr, Campinas, SP - Brazil
[5] Empresa Brasileira Pesquisa Agr, Embrapa Pesca & Aquicultura, Palmas, TO - Brazil
Total Affiliations: 5
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 43, n. 4 2020.
Web of Science Citations: 0
Abstract

In the present study, the complete characterization of cDNA and genomic sequences of IL-1 beta and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1 beta cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1 beta, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive m RNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1 beta and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pm IL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups. (AU)

FAPESP's process: 17/26996-6 - Evaluation of the tripliody in the susceptibility to the infection of Aeromonas jandaei and possible relation with the expression levels of immune related genes in lambaris (Astyanax mexicanus)
Grantee:Antonio Augusto Mendes Maia
Support Opportunities: Regular Research Grants
FAPESP's process: 17/18562-6 - Immunization of lambari (Astyanax altiparanae) with a DNA vaccine containing the immobilization antigen IAG52A from Ichthyophthirius multifiliis with the cytokine IL-8 from A. altiparanae as molecular adjuvant
Grantee:Mateus Maldonado Carriero
Support Opportunities: Scholarships in Brazil - Post-Doctoral