Dorsal hippocampus plays a causal role in context-induced reinstatement of alcohol-seeking in rats

Autor(es):Mostrar menos -

Felipe, Jaqueline Moreira ^[1] ; Palombo, Paola ^[1] ; Bianchi, Paula Cristina ^[1] ; Zaniboni, Caroline Riberti ^[1] ; Anesio, Augusto ^[1] ; Yokoyama, Thais Suemi ^[1] ; Engi, Sheila Antonagi ^[1] ; Carneiro-de-Oliveira, Paulo Eduardo ^[2] ; Planeta, Cleopatra da Silva ^[3] ; Leao, Rodrigo Molini ^[4] ; Cruz, Fabio Cardoso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Pharmacol, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Carlos UFSCar, Psychol Lab, Dept Psychol, BR-13565905 Sao Carlos, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Pharmacol Lab, Sch Pharmaceut Sci, Araraquara, SP - Brazil

^[4] Univ Fed Uberlandia UFU, Inst Biomed Sci, BR-38400902 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Behavioural Brain Research; v. 398, FEB 1 2021.
• Citações Web of Science: 1

Resumo

Drug addiction is a chronic mental disorder characterized by frequent relapses. Contextual cues associated with drug use to play a critical causal role in drug-seeking behavior. The hippocampus has been implicated in encoding drug associative memories. Here we examine whether the dorsal hippocampus mediates context induced reinstatement of alcohol-seeking. Male Long-Evans rats were trained to self-administer alcohol in Context A. Alcohol self-administration was extinguished in a distinct context (Context B). On the test day, animals were re-exposed to the alcohol Context A or the extinction Context B. Next, to assess a causal role for the dorsal hippocampus in context-induced alcohol-seeking, on the test day, we injected cobalt chloride (CoCl2; a nonselective synapse inhibitor) or vehicle into the dorsal hippocampus, and 15 min later, rats were tested by re exposing them to the drug-associated context. The re-exposure to the alcohol-associated Context A reinstated alcohol seeking and increased Fos-positive cells in the dorsal hippocampus neurons (CA1, CA3, and Dentate Gyrus). Pharmacological inactivation with cobalt chloride of the dorsal hippocampus attenuated the reinstatement of alcohol-seeking. Our data suggest that the dorsal hippocampus may be involved in context-induced alcohol-seeking behavior. (AU)

• Processo FAPESP: 18/15505-4 - Estudo neurobiológico da recaída ao uso de cocaína e crack: identificação de plasticidades em neuronal ensembles que armazenam memórias relacionadas com a adição de drogas
• Beneficiário: Fabio Cardoso Cruz
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2