Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii

Autor(es)

Rodrigues, Anderson Messias ^[1] ; Kubitschek-Barreira, Paula Helena ^[2] ; Pinheiro, Breno Goncalves ^[1] ; Teixeira-Ferreira, Andre ^[3] ; Hahn, Rosane Christine ^{[4, 5]} ; de Camargo, Zoilo Pires ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Discipline Cellular Biol, Lab Emerging Fungal Pathogens, Dept Microbiol Immunol & Parasitol, UNIFESP, BR-04023062 Sao Paulo - Brazil

^[2] Rio de Janeiro State Univ UERJ, Roberto Alcantara Gomes Inst Biol, Dept Cellular Biol, BR-20511010 Rio De Janeiro - Brazil

^[3] Fiocruz MS, Toxinol Lab, Dept Physiol & Pharmacodynam, BR-21040900 Rio De Janeiro - Brazil

^[4] Univ Fed Mato Grosso, Fac Med, Lab Mycol Res, BR-78060900 Cuiaba - Brazil

^[5] Univ Fed Mato Grosso, Julio Muller Univ Hosp, BR-78048902 Cuiaba - Brazil

^[6] Univ Fed Sao Paulo, Dept Med, Discipline Infect Dis, UNIFESP, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FUNGI; v. 6, n. 4 DEC 2020.
• Citações Web of Science: 0

Resumo

Paracoccidioidomycosis (PCM) is a life-threatening systemic infection caused by the fungal pathogen Paracoccidioides brasiliensis and related species. Whole-genome sequencing and stage-specific proteomic analysis of Paracoccidioides offer the opportunity to profile humoral immune responses against P. lutzii and P. brasiliensis s. str. infection using innovative screening approaches. Here, an immunoproteomic approach was used to identify PCM-associated antigens that elicit immune responses by combining 2-D electrophoresis of P. lutzii and P. brasiliensis proteomes, immunological detection using a gold-standard serum, and mass spectrometry analysis. A total of 16 and 25 highly immunoreactive proteins were identified in P. lutzii and P. brasiliensis, respectively, and 29 were shown to be the novel antigens for Paracoccidioides species, including seven uncharacterized proteins. Among the panel of proteins identified, most are involved in metabolic pathways, carbon metabolism, and biosynthesis of secondary metabolites in both immunoproteomes. Remarkably, six isoforms of the surface-associated enolase in the range of 54 kDa were identified as the major antigens in human PCM due to P. lutzii. These novel immunoproteomes of Paracoccidioides will be employed to develop a sensitive and affordable point-of-care diagnostic assay and an effective vaccine to identify infected hosts and prevent infection and development of human PCM. These findings provide a unique opportunity for the refinement of diagnostic tools of this important neglected systemic mycosis, which is usually associated with poverty. (AU)

• Processo FAPESP: 18/21460-3 - Estudo de diferentes preparações antigênicas de Paracoccidioides lutzii para a padronização do teste de ELISA como auxílio no diagnóstico da paracoccidioidomicose por P. lutzii
• Beneficiário: Zoilo Pires de Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/27265-5 - Epidemiologia molecular e perspectivas genômicas na evolução e propagação de patógenos fúngicos emergentes
• Beneficiário: Anderson Messias Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores