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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii

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Author(s):
Rodrigues, Anderson Messias [1] ; Kubitschek-Barreira, Paula Helena [2] ; Pinheiro, Breno Goncalves [1] ; Teixeira-Ferreira, Andre [3] ; Hahn, Rosane Christine [4, 5] ; de Camargo, Zoilo Pires [1, 6]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Discipline Cellular Biol, Lab Emerging Fungal Pathogens, Dept Microbiol Immunol & Parasitol, UNIFESP, BR-04023062 Sao Paulo - Brazil
[2] Rio de Janeiro State Univ UERJ, Roberto Alcantara Gomes Inst Biol, Dept Cellular Biol, BR-20511010 Rio De Janeiro - Brazil
[3] Fiocruz MS, Toxinol Lab, Dept Physiol & Pharmacodynam, BR-21040900 Rio De Janeiro - Brazil
[4] Univ Fed Mato Grosso, Fac Med, Lab Mycol Res, BR-78060900 Cuiaba - Brazil
[5] Univ Fed Mato Grosso, Julio Muller Univ Hosp, BR-78048902 Cuiaba - Brazil
[6] Univ Fed Sao Paulo, Dept Med, Discipline Infect Dis, UNIFESP, BR-04023062 Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: JOURNAL OF FUNGI; v. 6, n. 4 DEC 2020.
Web of Science Citations: 0
Abstract

Paracoccidioidomycosis (PCM) is a life-threatening systemic infection caused by the fungal pathogen Paracoccidioides brasiliensis and related species. Whole-genome sequencing and stage-specific proteomic analysis of Paracoccidioides offer the opportunity to profile humoral immune responses against P. lutzii and P. brasiliensis s. str. infection using innovative screening approaches. Here, an immunoproteomic approach was used to identify PCM-associated antigens that elicit immune responses by combining 2-D electrophoresis of P. lutzii and P. brasiliensis proteomes, immunological detection using a gold-standard serum, and mass spectrometry analysis. A total of 16 and 25 highly immunoreactive proteins were identified in P. lutzii and P. brasiliensis, respectively, and 29 were shown to be the novel antigens for Paracoccidioides species, including seven uncharacterized proteins. Among the panel of proteins identified, most are involved in metabolic pathways, carbon metabolism, and biosynthesis of secondary metabolites in both immunoproteomes. Remarkably, six isoforms of the surface-associated enolase in the range of 54 kDa were identified as the major antigens in human PCM due to P. lutzii. These novel immunoproteomes of Paracoccidioides will be employed to develop a sensitive and affordable point-of-care diagnostic assay and an effective vaccine to identify infected hosts and prevent infection and development of human PCM. These findings provide a unique opportunity for the refinement of diagnostic tools of this important neglected systemic mycosis, which is usually associated with poverty. (AU)

FAPESP's process: 18/21460-3 - Study of different antigenic preparations of Paracoccidioides lutzii for the standardization of the ELISA test as an aid in the diagnosis of paracoccidioidomycosis due to P. lutzii
Grantee:Zoilo Pires de Camargo
Support Opportunities: Regular Research Grants
FAPESP's process: 17/27265-5 - Molecular epidemiology and genomic perspectives on the evolution and spread of emerging fungal pathogens
Grantee:Anderson Messias Rodrigues
Support Opportunities: Research Grants - Young Investigators Grants