Canine brucellosis is a zoonotic disease, which causes economic losses to dog breeders owing to the reproductive disorders. It is a neglected bacterial infection caused by Brucella canis that is endemic in Brazil. The diagnosis of the infection is hard since that is no accurate serological tests, especially to identificate chronically infected dogs. The aim of this study is to perform a proteomic analysis of a field B. canis strain isolated in Brazil in order to identify immunogenic proteins to be used as antigens for the infection serodiagnosis. A sera panel obtained from 17 infected dogs sampled in different phases of infection (bacteremic and non-bacteremic) will be used, as well as sera obtained from five non-infected dogs to be used as control. The protein extraction of the B. canis strain followed by protein separation using two-dimensional electrophoresis will be performed. The protein profile obtained will be immunoblotted using the panel of canine sera to identify reactive proteins that are markers of the different stages of infection. The selected proteins will be identified using mass spectrometry by MALDI-TOF-MS technique.
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