Evaluation of different serological tests to the diagnosis of canine brucellosis caused by Brucella canis in naturally infected dogs

• Grant number: 10/52298-5
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2011
• End date: January 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Lara Borges Keid
• Grantee: Lara Borges Keid
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

Canine brucellosis caused by Brucella canis is chronic infection that affects the reproductive tract of dogs. The infection is important for commercial breeding kennels because once it is introduced in a confined canine population it can reach high prevalence rates, leading to economic losses. Canine brucellosis is a zoonotic infection and it is a public health concern, because of the close contact that is established between dogs and human beings. There is no vaccine to be used to prevent canine brucellosis in dogs and the antibiotic treatment of the infection is usually inefficient. Therefore, the laboratory diagnosis comprises an important tool to the control and prevention of the infection because it allows the identification and isolation of the infected animals. The serological tests most commonly used for the diagnosis of canine brucellosis are the rapid slide agglutination test with and without the use of the 2-mercapthoetanol (RSAT and 2ME-RSAT), and the agar gel immunodiffusion test (AGID). However, these serological tests showed a low diagnostic sensitivity and specificity, which difficult the infection diagnosis and compromise the implementation of prophylactic measures. Other serological tests have been used for the diagbnosis of this infection with variable performances like the enzyme-linked immunosorbent assay in liquid and in solid phase and the indirect immunofluorescence reaction (M). The objective of this project is to standardize an indirect ELISA test using the BP26 recombinant antigen from B. abortus to the diagnosis of canine brucellosis and to evaluate the performance of commercial kits used for the serological diagnosis of the infection. The performance of the indirect ELISA and the commercial kits will be compared to the performance of the RSAT, 2ME-RSAT, AGID, bacteriological culture and polymerase chain reaction. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KEID, L. B.; DINIZ, J. A.; OLIVEIRA, T. M. F. S.; FERREIRA, H. L.; SOARES, R. M.. Evaluation of an Immunochromatographic Test to the Diagnosis of Canine Brucellosis Caused by Brucella canis. REPRODUCTION IN DOMESTIC ANIMALS, v. 50, n. 6, p. 939-944, DEC 2015. (10/52298-5)