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Molecular epidemiology of abortions and reproductive problems of infectious causes in dogs with emphasis in canine herpesvírus

Grant number: 11/04795-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): June 30, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:João Pessoa Araújo Junior
Grantee:Jacqueline Kazue Kurissio
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The canine herpesvirus type 1 (CHV-1) belongs to family Herpesviridae, subfamily Alphaherpesvirinae and member of the genus Varicellovirus acts in the reproductive sphere is considered enzootic in the dog population in various parts of the world. It accounts for abortions and reproductive problems in female dogs, and even its ability to increase its latency and persistence in animal transmission to other animals. Besides Herpesvirus other infectious agents are involved in reproductive failure in female dogs as brucella canis, Leptospira spp. and Toxoplasma gondii, which have great importance due to its zoonotic potential, however, in cases of reproductive failure in dogs there are no surveys regarding the causes of abortion and its population, making it difficult to determine the status and causes of reproductive problems affected this species and activities of agents. In Brazil, there is no work reported on the standardization of the technique or methodology applied to molecular diagnosis using qPCR for canine herpes virus, so far. Therefore, this paper aims to standardize the qPCR technique as a tool for the diagnosis and choice for canine herpesvirus, in a sensitive, specific and efficient. Even if it's already happened abroad, reagents and equipment used are different and the possibility of genetic variability of CHV-1 justifies the standardization of the technique in our midst. Still, this work aims to conduct an epidemiological survey of infectious agents of major causes of abortion in dogs. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
KURISSIO, Jacqueline Kazue. Isolamento viral e diagnóstico molecular de herpevírus canino. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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