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Study of different antigenic preparations of Paracoccidioides lutzii for the standardization of the ELISA test as an aid in the diagnosis of paracoccidioidomycosis due to P. lutzii

Grant number: 18/21460-3
Support type:Regular Research Grants
Duration: August 01, 2019 - July 31, 2021
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Zoilo Pires de Camargo
Grantee:Zoilo Pires de Camargo
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Assoc. researchers:Anderson Messias Rodrigues ; Eva Burger ; Olga Fischman Gompertz ; Rosane Christine Hahn


Traditionally, paracoccidioidomycosis (PCM) is known to be a deep mycosis caused by the thermodymorphic fungus Paracoccidioides brasiliensis. However, there are indications of other species of Paracoccidioides. One such species is P. lutzii, previously named Pb01-like. The other species are P. brasiliensis S1, PS2, PS3 and PS4, recently named P. brasiliensis sensu stricto, P. americana, P. restrepiensis and P. venezuelensis, respectively. The disease is limited to Latin America countries and very little is known about their ecologies. The highest incidence of P. lutzii is in the center-west region of Brazil; however, it may occur in other regions as well. The diversity of clinical forms of the disease is related to the host's immune status and intrinsic characteristics of the fungus. Antigenic proteins may be used as serological markers in the development of more specific diagnostic tests. Conventional serological tests, such as immunodiffusion, using P. brasiliensis exoantigen cannot diagnose PCM due P. lutzii. In this project, we intend to standardize the ELISA test to aid in the diagnosis of PCM by P. lutzii. For this purpose, several types of antigenic preparations derived from P. lutzii will be tested using sera from PCM patients due to P. lutzii and P. brasiliensis. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PINHEIRO, BRENO GONCALVES; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives. JOURNAL OF FUNGI, v. 6, n. 4 DEC 2020. Web of Science Citations: 1.
RODRIGUES, ANDERSON MESSIAS; KUBITSCHEK-BARREIRA, PAULA HELENA; PINHEIRO, BRENO GONCALVES; TEIXEIRA-FERREIRA, ANDRE; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES. Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii. JOURNAL OF FUNGI, v. 6, n. 4 DEC 2020. Web of Science Citations: 0.
RODRIGUES, A. M.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; TERRA, P. P. D.; DE HOOG, S.; BRILHANTE, R. S. N.; DE AGUIAR CORDEIRO, R.; DE SOUZA COLLARES MAIA CASTELO-BRANCO, D.; ROCHA, M. F. G.; SIDRIM, J. J. C.; DE CAMARGO, Z. P. The global epidemiology of emerging Histoplasma species in recent years. STUDIES IN MYCOLOGY, n. 97 SEP 2020. Web of Science Citations: 5.
DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; FISHER, MATTHEW C.; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Genome-wide mapping using new AFLP markers to explore intraspecific variation among pathogenicSporothrixspecies. PLoS Neglected Tropical Diseases, v. 14, n. 7 JUL 2020. Web of Science Citations: 0.
RODRIGUES, ANDERSON MESSIAS; DELLA TERRA, PAULA PORTELLA; GREMIAO, ISABELLA DIB; PEREIRA, SANDRO ANTONIO; OROFINO-COSTA, ROSANE; DE CAMARGO, ZOILO PIRES. The threat of emerging and re-emerging pathogenic Sporothrix species. Mycopathologia, v. 185, n. 5, SI FEB 2020. Web of Science Citations: 10.

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