Molecular epidemiology and genomic perspectives on the evolution and spread of emerging fungal pathogens

• Grant number: 17/27265-5
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: October 01, 2018
• End date: September 30, 2024
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Anderson Messias Rodrigues
• Grantee: Anderson Messias Rodrigues
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Ana Luisa Höfling-Lima ; Arnaldo Lopes Colombo ; Ferry Hagen ; Jesus Guinea ; João Nóbrega de Almeida Júnior ; Matthew Fisher ; Matthias Brock ; Roberta Peres da Silva ; Sybren de Hoog ; Zoilo Pires de Camargo
• Associated research grant(s): 23/12272-7 - Multi-user equipment approved in grant 2017/27265-5: QuantStudio real-time PCR system, AP.EMU; 18/24304-2 - Multi-user equipament approved in grant 2017/27265-5: SeqStudio Ggnetic analyzer, AP.EMU
• Associated scholarship(s): 21/10364-6 - Application of MALDI-ToF mass spectrometry to identify classical and emerging agents of Fusarium spp, BP.IC; 21/03018-4 - Maintenance of medically relevant fungal agents using classical and molecular identification., BP.TT; 21/02326-7 - Development of new molecular tools for the identification of medically relevant Fusarium spp., BP.MS; 21/02327-3 - Analysis of genetic diversity in aspergillosis agents using AFLP (Amplified Fragment Length Polymorphism) and microsatellites markers, BP.MS; 19/14799-7 - Molecular epidemiology and genomic perspectives on the evolution and spread of emerging fungal pathogens, BP.TT

Abstract

Fungal diseases kill more than 1.5 million individuals and affect more than one billion people annually around the world. These infections are still neglected by public health authorities, although most deaths are preventable. In this project, we propose to explore emerging pathogens among some of the main Brazilian mycoses, including Aspergillosis, Fusariosis, Sporotrichosis, and Paracoccidioidomycosis, integrating study systems (epidemiology, diagnosis, and genomics), expanding national and international networking. Our aims are to identify, diagnose and understand how fungal epidemics develop. The first topic of this project uses molecular epidemiology methods to investigate the pathogen-host-environment interplay, disease transmission pathways, and disease emergence. Key issues such as epidemiology, population structure, genetic diversity and host association will be extensively explored. The second topic aims to identify new genetic and proteomic markers that can be used in the early diagnosis of fungal infections using as the main strategy the detection of a marker in tissue samples and biological fluids. Diagnostic tests are essential for patient follow-up and disease control. Misdiagnosis or lack there of results in public health problems, whether due to failure to treat or inadequate therapy. The third topic employs next-generation sequencing and comparative genomics for molecular typing as well as understanding the evolution and dispersion of fungal epidemics, making it possible to track current outbreaks and epidemics in Brazil. These pieces of information provide a unique opportunity to study the biology and evolution of medically-relevant fungi. Our experimental approach is a first step towards recognizing epidemiological and evolutionary trajectories of pathogens aiming to propose better public health policies to contain fungal epidemics and therefore reduce the number of infections in animals and humans or prevent the emergence of new pathogens. (AU)

Scientific publications

(35)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, A. M.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; TERRA, P. P. D.; DE HOOG, S.; BRILHANTE, R. S. N.; DE AGUIAR CORDEIRO, R.; DE SOUZA COLLARES MAIA CASTELO-BRANCO, D.; ROCHA, M. F. G.; et al. The global epidemiology of emerging Histoplasma species in recent years. STUDIES IN MYCOLOGY, n. 97, SEP 2020. (17/27265-5, 18/21460-3)

RODRIGUES, ANDERSON MESSIAS; DELLA TERRA, PAULA PORTELLA; GREMIAO, ISABELLA DIB; PEREIRA, SANDRO ANTONIO; OROFINO-COSTA, ROSANE; DE CAMARGO, ZOILO PIRES. The threat of emerging and re-emerging pathogenic Sporothrix species. Mycopathologia, v. 185, n. 5, SI, FEB 2020. (17/27265-5, 18/21460-3)

RODRIGUES, ANDERSON MESSIAS; HAGEN, FERRY; PUCCIA, ROSANA; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES. Paracoccidioides and Paracoccidioidomycosis in the 21st Century. Mycopathologia, v. N/A, p. 5-pg., 2023-01-12. (17/27265-5)

RODRIGUES, ANDERSON MESSIAS; HAGEN, FERRY; DE CAMARGO, ZOILO PIRES. A Spotlight on Sporothrix and Sporotrichosis. Mycopathologia, v. N/A, p. 5-pg., 2022-07-01. (17/27265-5)

MACEDO-SALES, PAMELLA ANTUNES; PEREIRA SOUZA, LUCIERI OLEGARIO; DELLA-TERRA, PAULA PORTELLA; LOZOYA-PEREZ, NANCY EDITH; DANTAS MACHADO, RICARDO LUIZ; DA SILVA DA ROCHA, ELISABETH MARTINS; LOPES-BEZERRA, LEILA MARIA; GUIMARAES, ALLAN JEFFERSON; RODRIGUES, ANDERSON MESSIAS; MANUEL MORA-MONTES, HECTOR; et al. Coinfection of domestic felines by distinct Sporothrix brasiliensis in the Brazilian sporotrichosis hyperendemic area. Fungal Genetics and Biology, v. 140, JUL 2020. (17/27265-5)

PINHEIRO, BRENO GONCALVES; POSSA, ANA PAULA; DELLA TERRA, PAULA PORTELLA; DE CARVALHO, JAMILE AMBROSIO; RICCI, GIANNINA; NISHIKAKU, ANGELA SATIE; HAHN, ROSANE CHRISTINE; CAMARGO, ZOILO PIRES DE; RODRIGUES, ANDERSON MESSIAS. A New Duplex PCR-Assay for the Detection and Identification of Paracoccidioides Species. JOURNAL OF FUNGI, v. 7, n. 3, MAR 2021. (17/27265-5, 18/21460-3)

DELLA TERRA, PAULA PORTELLA; GONSALES, FERNANDA FIDELIS; DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; KANO, RUI; BONIFAZ, ALEXANDRO; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Development and evaluation of a multiplex qPCR assay for rapid diagnostics of emerging sporotrichosis. TRANSBOUNDARY AND EMERGING DISEASES, NOV 2021. (18/21460-3, 17/27265-5)

MAIFREDE, SIMONE BRAVIM; KRUSCHEWSKY, WDSON LUIS LIMA; PATRICIO, SUZANA ALVES; FALQUETO, ALOISIO; PECANHA, PAULO MENDES; MALAQUIAS, LUIZ COSME COTTA; POSSA, ANA PAULA; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; GONCALVES, SARAH SANTOS; et al. Screening paracoccidioidomycosis by double immunodiffusion test in a referred diagnostic center in Brazilian southeastern: an accessible tool. INFECTION, v. 49, n. 6, SEP 2021. (18/21460-3, 17/27265-5)

PECANHA, PAULO MENDES; BAHIENSE, ISABELA CRUZ; LIMA KRUSCHEWSKY, WDSON LUIS; BIASUTTI, CLAUDIA; GONCALVES FERREIRA JUNIOR, CARLOS URBANO; PINHEIRO, BRENO GONCALVES; MAIFREDE, SIMONE BRAVIM; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; GRAO-VELLOSO, TANIA REGINA; et al. Paracoccidioidomycosis due to Paracoccidioides brasiliensis S1 associated with acquired immunodeficiency syndrome: A case report. REVISTA IBEROAMERICANA DE MICOLOGIA, v. 38, n. 1, p. 5-8, JAN-MAR 2021. (17/27265-5)

HAHN, ROSANE CHRISTINE; RODRIGUES, ANDERSON MESSIAS; DELLA TERRA, PAULA PORTELLA; NERY, ANDREIA FERREIRA; HOFFMANN-SANTOS, HUGO DIAS; GOIS, HELLEN MEIRA; FERNANDES FONTES, COR JESUS; DE CAMARGO, ZOILO PIRES. Clinical and epidemiological features of paracoccidioidomycosis due to Paracoccidioides lutzii. PLoS Neglected Tropical Diseases, v. 13, n. 6, JUN 2019. (09/54024-2, 17/27265-5)

PINHEIRO, BRENO GONCALVES; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives. JOURNAL OF FUNGI, v. 6, n. 4, DEC 2020. (17/27265-5, 18/21460-3)

ROBERTO, T. N.; DE CARVALHO, J. A.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; HAHN, R. C.; DE CAMARGO, Z. P.; RODRIGUES, A. M.. xploring genetic diversity, population structure, and phylogeography in Paracoccidioides species using AFLP marker. STUDIES IN MYCOLOGY, n. 100, SEP 2021. (13/05405-9, 17/27265-5, 18/21460-3)

MASCHIO-LIMA, TAIZA; RIBEIRO MARQUES, MARIELA DOMICIANO; LEMES, THIAGO HENRIQUE; BRIZZOTTI-MAZUCHI, NATALIA SERON; CAETANO, MAICON HENRIQUE; DE ALMEIDA, BIANCA GOTTARDO; BIANCO, LETICIA MONTEIRO; MONTEIRO, RUAN CAMPOS; RODRIGUES, ANDERSON MESSIAS; DE CAMARGO, ZOILO PIRES; et al. Clinical and epidemiological aspects of feline sporotrichosis caused by Sporothrix brasiliensis and in vitro antifungal susceptibility. VETERINARY RESEARCH COMMUNICATIONS, v. 45, n. 4, p. 171-179, DEC 2021. (17/27265-5)

RIBEIRO-MARQUES, MARIELA DOMICIANO; MASCHIO-LIMA, TAIZA; LEMES, THIAGO HENRIQUE; SIQUEIRA, JOAO PAULO ZEN; BRIZZOTTI-MAZUCHI, NATALIA SERON; CAETANO, MAICON HENRIQUE; ALMEIDA, BIANCA GOTTARDO; MOZANER, LETICIA QUEIROZ; MONTEIRO, RUAN CAMPOS; CAMARGO, ZOILO PIRES; et al. Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates. Medical Mycology, v. 61, n. 1, p. 5-pg., 2022-12-29. (17/27265-5)

GUEVARA, ARMANDO; NERY, ANDREIA FERREIRA; CARVALHO MELHEM, MARCIA DE SOUZA; BONFIETTI, LUCAS; RODRIGUES, ANDERSON MESSIAS; HAGEN, FERRY; DE CARVALHO, JAMILE AMBROSIO; DE CAMARGO, ZOILO PIRES; DE SOUZA LIMA, BRUNA JACOMEL F.; VICENTE, VANIA APARECIDA; et al. Molecular epidemiology and clinical-laboratory aspects of chromoblastomycosis in Mato Grosso, Brazil. MYCOSES, v. 65, n. 12, p. 13-pg., 2022-09-29. (18/24304-2, 17/27265-5)

HAHN, ROSANE CHRISTINE; HAGEN, FERRY; MENDES, RINALDO PONCIO; BURGER, EVA; NERY, ANDREIA FERREIRA; SIQUEIRA, NATHAN PEREIRA; GUEVARA, ARMANDO; RODRIGUES, ANDERSON MESSIAS; DE CAMARGO, ZOILO PIRES. Paracoccidioidomycosis: Current Status and Future Trends. CLINICAL MICROBIOLOGY REVIEWS, v. 35, n. 4, p. 65-pg., 2022-09-08. (17/27265-5, 18/21460-3)

RODRIGUES, A. M.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; TERRA, P. P. D.; DE HOOG, S.; BRILHANTE, R. S. N.; DE AGUIAR CORDEIRO, R.; DE SOUZA COLLARES MAIA CASTELO-BRANCO, D.; ROCHA, M. F. G.; et al. The global epidemiology of emerging Histoplasma species in recent years. STUDIES IN MYCOLOGY, v. N/A, n. 97, p. 21-pg., 2020-09-01. (18/21460-3, 17/27265-5)

DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; FISHER, MATTHEW C.; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Genome-wide mapping using new AFLP markers to explore intraspecific variation among pathogenicSporothrixspecies. PLoS Neglected Tropical Diseases, v. 14, n. 7, JUL 2020. (18/21460-3, 17/27265-5)

MASCHIO-LIMA, TAIZA; RIBEIRO MARQUES, MARIELA DOMICIANO; LEMES, THIAGO HENRIQUE; BRIZZOTTI-MAZUCHI, NATALIA SERON; CAETANO, MAICON HENRIQUE; DE ALMEIDA, BIANCA GOTTARDO; BIANCO, LETICIA MONTEIRO; MONTEIRO, RUAN CAMPOS; RODRIGUES, ANDERSON MESSIAS; DE CAMARGO, ZOILO PIRES; et al. Clinical and epidemiological aspects of feline sporotrichosis caused by Sporothrix brasiliensis and in vitro antifungal susceptibility. VETERINARY RESEARCH COMMUNICATIONS, JUN 2021. (17/27265-5)

NERY, ANDREIA FERREIRA; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; PORTELA, TIAGO FERREIRA; HOFFMANN-SANTOS, HUGO DIAS; KRUGER DAMBROS, PEDRO VITOR; RIBEIRO DE SOUZA, JANIO FELIPE; GARCIA, ALEXANDRE CARVALHO; DAMASIO SANTOS, CAROLINA ARAUJO; HAGEN, FERRY; et al. Paracoccidioidomycosis due to P lutzii: The importance of neutrophil/lymphocyte ratio in the symptomatic and asymptomatic phases in severe cases. MYCOSES, v. 64, n. 8, p. 874-881, AUG 2021. (17/27265-5, 18/21460-3)

DE CARVALHO, J. A.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; KANO, R.; BONIFAZ, A.; TORIELLO, C.; NEGRONI, R.; REGO, R. S. DE M.; GREMIAO, I. D. F.; et al. rends in the molecular epidemiology and population genetics of emerging Sporothrix specie. STUDIES IN MYCOLOGY, n. 100, SEP 2021. (17/27265-5, 18/21460-3)

DO AMARAL, CRISTIANE CANDIDA; FERNANDES, GEISA FERREIRA; RODRIGUES, ANDERSON MESSIAS; BURGER, EVA; DE CAMARGO, ZOILO PIRES. Proteomic analysis of Paracoccidioides brasiliensis complex isolates: Correlation of the levels of differentially expressed proteins with in vivo virulence. PLoS One, v. 14, n. 7, JUL 2 2019. (09/54024-2, 17/27265-5)

RODRIGUES, ANDERSON MESSIAS; KUBITSCHEK-BARREIRA, PAULA HELENA; PINHEIRO, BRENO GONCALVES; TEIXEIRA-FERREIRA, ANDRE; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES. Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii. JOURNAL OF FUNGI, v. 6, n. 4, DEC 2020. (18/21460-3, 17/27265-5)

NERY, ANDREIA FERREIRA; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; PORTELA, TIAGO FERREIRA; HOFFMANN-SANTOS, HUGO DIAS; PINHEIRO, BRENO GONCALVES; POSSA, ANA PAULA; DA SILVA CAVALCANTE, LETICIA ROSSETTO; HAGEN, FERRY; HAHN, ROSANE CHRISTINE. Puzzling paracoccidioidomycosis: Factors associated with the severity of Paracoccidioides lutzii infections. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 107, p. 284-290, JUN 2021. (17/27265-5, 18/21460-3)

DE CARVALHO, JAMILE AMBROSIO; PINHEIRO, BRENO GONCALVES; HAGEN, FERRY; GONCALVES, SARAH SANTOS; NEGRONI, RICARDO; KANO, RUI; BONIFAZ, ALEXANDRO; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. A new duplex PCR assay for the rapid screening of mating-type idiomorphs of pathogenic Sporothrix species. FUNGAL BIOLOGY, v. 125, n. 10, p. 834-843, OCT 2021. (17/27265-5)

RODRIGUES, ANDERSON MESSIAS; GONCALVES, SARAH SANTOS; DE CARVALHO, JAMILE AMBROSIO; BORBA-SANTOS, LUANA P.; ROZENTAL, SONIA; DE CAMARGO, ZOILO PIRES. Current Progress on Epidemiology, Diagnosis, and Treatment of Sporotrichosis and Their Future Trends. JOURNAL OF FUNGI, v. 8, n. 8, p. 32-pg., 2022-08-01. (17/27265-5)

BAHIENSE ROCHA, ISABELA DA CRUZ; DELLA TERRA, PAULA PORTELLA; DE OLIVEIRA, RODRIGO CARDOSO; ZANOTTI, RAPHAEL LUBIANCA; FALQUETO, ALOISIO; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; GONCALVES, SARAH SANTOS. Molecular-based assessment of diversity and population structure of Sporothrix spp. clinical isolates from Espirito Santo-Brazil. MYCOSES, v. 64, n. 4, DEC 2020. (17/27265-5)

REDIGUIERI, BRUNO CARNEIRO; BAHIENSE, ISABELA DA CRUZ; DE CARVALHO, JAMILE AMBROSIO; LEITE, GUSTAVO ROCHA; FALQUETO, ALOISIO; RODRIGUES, ANDERSON MESSIAS; GONCALVES, SARAH SANTOS. Clinical, Epidemiological, and Epizootic Features of Sporothrix brasiliensis in Espirito Santo, Brazil. ECOHEALTH, v. 19, n. 1, p. 11-pg., 2022-02-21. (17/27265-5)

ALVES, MANOELLA DO MONTE; MILAN, EVELINE PIPOLO; DA SILVA-ROCHA, WALICYRANISON PLINIO; DE SENA DA COSTA, ALEXANDRE SOARES; MACIELID, BRUNO ARAUJO; CAVALCANTE VALE, PEDRO HENRIQUE; DE ALBUQUERQUE, PAULO ROBERTO; LIMA, SORAIA LOPES; DE AZEVEDO MELO, ANALY SALLES; RODRIGUES, ANDERSON MESSIAS; et al. Fatal pulmonary sporotrichosis caused by Sporothrix brasiliensis in Northeast Brazil. PLoS Neglected Tropical Diseases, v. 14, n. 5, MAY 2020. (17/27265-5)

DE CARVALHO, JAMILE AMBROSIO; MONTEIRO, RUAN CAMPOS; HAGEN, FERRY; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Trends in Molecular Diagnostics and Genotyping Tools Applied for Emerging Sporothrix Species. JOURNAL OF FUNGI, v. 8, n. 8, p. 30-pg., 2022-08-01. (17/27265-5)

DE MACEDO, PRISCILA MARQUES; STURNY-LECLERE, AUDE; FREITAS, DAYVISON FRANCIS SARAIVA; GHELFENSTEIN-FERREIRA, THEO; GUTIERREZ-GALHARDO, MARIA CLARA; ALMEIDA, MARCOS DE ABREU; RODRIGUES, ANDERSON MESSIAS; PAUTET, THIERRY; HAMANE, SAMIA; ALMEIDA-PAES, RODRIGO; et al. Development and validation of a new quantitative reverse transcription PCR assay for the diagnosis of human sporotrichosis. Medical Mycology, v. 61, n. 7, p. 7-pg., 2023-07-06. (17/27265-5)

LOSADA, LUIZA CHAVES DE MIRANDA LEONHARDT; MONTEIRO, RUAN CAMPOS; DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; FISHER, MATTHEW C.; SPRUIJTENBURG, BRAM; MEIS, JACQUES F.; DE GROOT, THEUN; GONCALVES, SARAH SANTOS; NEGRONI, RICARDO; et al. High-Throughput Microsatellite Markers Development for Genetic Characterization of Emerging Sporothrix Species. JOURNAL OF FUNGI, v. 9, n. 3, p. 34-pg., 2023-03-01. (18/21460-3, 17/27265-5)

DE CARVALHO, JAMILE AMBROSIO; HAGEN, FERRY; FISHER, MATTHEW C.; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS. Genome-wide mapping using new AFLP markers to explore intraspecific variation among pathogenic Sporothrix species. PLoS Neglected Tropical Diseases, v. 14, n. 7, p. 26-pg., 2020-07-01. (18/21460-3, 17/27265-5)

RODRIGUES, ANDERSON MESSIAS; DELLA TERRA, PAULA PORTELLA; GREMIAO, ISABELLA DIB; PEREIRA, SANDRO ANTONIO; OROFINO-COSTA, ROSANE; DE CAMARGO, ZOILO PIRES. The threat of emerging and re-emerging pathogenic Sporothrix species. Mycopathologia, v. 185, n. 5, p. 30-pg., 2020-02-12. (18/21460-3, 17/27265-5)

KRUSCHEWSKY, WDSON LUIS LIMA; PATRICIO, SUZANA ALVES; BAHIENSE, ISABELA CRUZ; MAIFREDE, SIMONE BRAVIM; POSSA, ANA PAULA; DE CAMARGO, ZOILO PIRES; RODRIGUES, ANDERSON MESSIAS; PECANHA, PAULO MENDES; FALQUETO, ALOISIO; GRAO-VELLOSO, TANIA REGINA; et al. Paracoccidioidomycosis cases by Paracoccidioides lutzii in southeastern Brazil. Brazilian Journal of Microbiology, v. N/A, p. 7-pg., 2023-06-08. (18/21460-3, 17/27265-5)