Gamma-oryzanol as a potential modulator of oxidative stress and inflammation via PPAR-y in adipose tissue: a hypothetical therapeutic for cytokine storm in COVID-19?

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Francisqueti-Ferron, Fabiane Valentini ^[1] ; Garcia, Jessica Leite ^[1] ; Togneri Ferron, Artur Junio ^[1] ; Nakandakare-Maia, Erika Tiemi ^[1] ; Gregolin, Cristina Schmitt ^[1] ; das Chagas Silva, Janaina Paixao ^[2] ; dos Santos, Klinsmann Carolo ^[1] ; Colombo Lo, Angelo Thompson ^[1] ; Siqueira, Juliana Silva ^[2] ; de Mattei, Leticia ^[1] ; de Paula, Bruno Henrique ^[2] ; Sarzi, Felipe ^[3] ; Vagula de Almeida Silva, Carol Cristina ^[1] ; Moreto, Fernando ^[1] ; Costa, Mariane Rovero ^[1] ; Ferreira, Ana Lucia A. ^[1] ; Minatel, Igor Otavio ^[2] ; Correa, Camila Renata ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Med Sch, UNESP, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Inst Biosci, UNESP, Botucatu, SP - Brazil

^[3] Botucatu Integrated Coll UNIFAC, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 520, JAN 15 2021.
• Citações Web of Science: 0

Resumo

The literature has reported a higher prevalence of negative clinical outcomes due to Coronavirus disease 19 (COVID-19) in obese individuals. This can be explained by the cytokine storm, result from the cytokine production from both obesity and viral infection. Gamma-oryzanol (gamma Oz) is a compound with anti-inflammatory and antioxidant activities. However, little is known about the gamma Oz action as a possible agonist of pemxisome proliferator-activated receptor gamma (PPAR-gamma). The aim of this study was to test the hypothesis that gamma Oz attenuates the cytokine storm by stimulating PPAR-gamma in the adipose tissue. Methods: Male Wistar rats were randomly divided into three experimental groups and fed ad libitum for 30 weeks with control diet (C, n = 6), high sugar-fat diet (HSF, n = 6) or high sugar-fat diet + gamma Oz (HSF + gamma Oz, n = 6). HSF groups also received water + sucrose (25%). The gamma Oz dose was 0.5% in the chow. Evaluation in animals included caloric intake, body weight, adiposity index, plasma triglycerides, and HOMA-IR. In adipose tissue was evaluated: PPAR-gamma gene and protein expression, inflammatory and oxidative stress parameters, and histological analysis. Results: Adipose tissue dysfunction was observed in HSF group, which presented remarkable PPAR-gamma underexpression and increased levels of cytokines, other inflammatory markers and oxidative stress. The yOz treatment prevented adipose tissue dysfunction and promoted PPAR-gamma overexpression. Conclusion: Natural compounds as gamma Oz can be considered a coadjutant therapy to prevent the cytokine storm in COVID-19 patients with obesity conditions. (AU)

• Processo FAPESP: 18/15294-3 - Influência da intervenção com gama-orizanol na prevenção da síndrome metabólica cardiorreanal de ratos obesos: participação da carbonilação protéica
• Beneficiário: Camila Renata Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/10626-0 - Inter-relação do gama-orizanol e ativação dos receptores Adipo- R1 e Adipo- R2: possível efeito na melhora da função renal associada à obesidade
• Beneficiário: Camila Renata Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Regular