Effect of berberine associated with photodynamic therapy in cell lines

Autor(es)

Oliveira, P. M. ^[1] ; Lopes, T. Z. ^[2] ; Tedesco, A. C. ^[3] ; Rahal, P. ^[2] ; Calmon, M. F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Pelotas UFPel, Fed Univ Pelotas, Ctr Desenvolvimento Tecnol CDTec, Technol Dev Ctr, Pelotas, RS - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Biol, Inst Biosci Humanities & Exact Sci IBILCE, S ao Jose Do Rio Preto - Brazil

^[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, Ctr Nanotechnol & Tissue Engn, Photobiol Photomed, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 32, DEC 2020.
• Citações Web of Science: 0

Resumo

Cervical cancer is a serious worldwide health problem. In view of the potentially harmful effects of current conventional therapies, photodynamic therapy may be an option as it is a minimally invasive therapy and can promote selective cytotoxic activity for neoplastic cells in the target tissue., Berberine (BBR) as an isolated molecule is a natural compound that has antineoplastic properties and potential action as a photosensitizer agent. The purpose of this study was to evaluate the use of berberine as a photosensitizer in photodynamic therapy (PDT) protocols and observe the effects produced by this association in cervical carcinoma cells and in immortalized keratinocytes. Incubation with 2.5 mu M berberine promoted less than 10 % of cellular death in both cell lines studied. In addition, by fluorescence microscopy, we demonstrated that berberine was internalized by the cells, and after a period of 48 h, it was still present in the intracellular environment preferentially localized in the cytoplasm. After photodynamic therapy using berberine as a photosensitizer and visible light activation at 447 (+/- 10) nm, we observed a phototoxic effect, which resulted in 19.84 % cell viability for Caski cells and 47.22 % cell viability for HaCaT. Treatment with berberine associated with photodynamic therapy promoted an increase in the production of reactive species of oxygen (ROS) and caspase-3 activity, indicating a preferential cell death mechanism by caspase-dependent apoptosis. Therefore, we demonstrated that berberine is an efficient photosensitizer and that its association with photodynamic therapy may be a potential anticancer treatment strategy for cervical cancer. (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/16660-5 - Estudo de nanoemulsões associadas a terapia fotodinâmica no tratamento do câncer de mama
• Beneficiário: Marilia de Freitas Calmon
• Modalidade de apoio: Auxílio à Pesquisa - Regular