Contribution of Complement System pathways to the killing of Leptospira spp.

Autor(es)

Okochi Alves da Silva, Priscilla Yuri ^[1] ; Midon, Leonardo Moura ^[1] ; Heinemann, Marcos Bryan ^[2] ; Vasconcelos, Dewton de Moraes ^[3] ; Barbosa, Angela Silva ^[4] ; Isaac, Lourdes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Av Prof Lineu Prestes 1730, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Med Vet & Anim Sci, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Clin Hosp, Fac Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Bacteriol Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microbes and Infection; v. 22, n. 10, p. 550-557, NOV-DEC 2020.
• Citações Web of Science: 0

Resumo

The Complement System (CS) plays an important role in the immune response against leptospirosis and can be activated by the Alternative and Lectin Pathways (Innate Immunity) and by the Classical Pathway (Acquired Immunity). Here we analyzed a broad range of nonpathogenic and pathogenic Leptospira strains considering their interaction with each CS pathway. We determined bacterial survival rate and CS protein deposition in the presence of purified proteins, specific component depleted sera and NHS treated with the chelating agents EDTA (inhibits all three activation pathways) or EGTA (inhibits the Classical and Lectin Pathways). We suggest that the Lectin and the Alternative Pathways have an important role to eliminate saprophytic leptospires since i) approximately 50% survival of both saprophytic strains was observed in the presence of MBL-deficient serum; ii) approximately 50% survival of Leptospira biflexa Patoc I was observed in the presence of NHS - EGTA and iii) C1q-depleted serum caused significant bacterial lysis. In all serovars investigated the deposition of C5-C9 proteins on saprophytic Leptospira strains was more pronounced when compared to pathogenic species confirming previous studies in the literature. No difference on C3 deposition was observed between nonpathogenic and pathogenic strains. In conclusion, Leptospira strains interact to different degrees with CS proteins, especially those necessary to form MAC, indicating that some strains and specific ligands could favor the binding of certain CS proteins. (C) 2020 Published by Elsevier Masson SAS on behalf of Institut Pasteur. (AU)

• Processo FAPESP: 17/12924-3 - Etiopatogênese da Leptospirose: contribuição do sistema complemento in vivo e in vitro para o controle da infecção e desencadeamento da resposta inflamatória tecidual: estudo de polimorfismos de genes do sistema complemento em pacientes com Leptospirose
• Beneficiário: Lourdes Isaac
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/18936-3 - Identificação de polimorfismo gênico do CFH de pacientes com leptospirose
• Beneficiário: Leonardo Moura Midon
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica