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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Isolation and Sequencing of Cu-, Fe-, and Zn-Binding Whey Peptides for Potential Neuroprotective Applications as Multitargeted Compounds

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Autor(es):
Caetano-Silva, Maria Elisa [1] ; Simabuco, Fernando Moreira [2] ; Neves Bezerra, Rosangela Maria [2] ; da Silva, Daniele Cristina [1] ; Barbosa, Eder Alves [3, 4] ; Moreira, Daniel Carneiro [4] ; Brand, Guilherme Dotto [3] ; de Souza de Almeida Leite, Jose Roberto [4] ; Bertoldo Pacheco, Maria Teresa [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] ITAL, Inst Food Technol, Ctr Food Sci & Qual, CCQA, BR-13070178 Campinas - Brazil
[2] Univ Estadual Campinas, UNICAMP, Multidisciplinary Lab Food & Hlth, LABMAS, Sch Appl Sci, FCA, BR-13484350 Limeira - Brazil
[3] Univ Brasilia, Lab Synth & Anal Biomol, Inst Chem, IQ, BR-70910900 Brasilia, DF - Brazil
[4] Univ Brasilia, Fac Med, Morphol Area, Res Ctr Morphol & Appl Immunol, NuPMIA, UnB, BR-70910900 Brasilia, DF - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Agricultural and Food Chemistry; v. 68, n. 44, p. 12433-12443, NOV 4 2020.
Citações Web of Science: 0
Resumo

This study aims to isolate metal-binding peptides and synthesize promising amino acid sequences to potentially act as neuroprotective compounds in the future, targeting different mechanisms. Fractions of whey metal-binding peptides (Cu, Fe, and Zn) isolated by immobilized metal affinity chromatography showed different amino acid profiles according to the metal. The Cu-binding peptides presented roughly twofold increase in the in vitro antioxidant, as assessed by oxygen radical absorbance capacity and anticholinesterase activities over the hydrolysate. This is probably because of the higher concentration of aromatic and basic residues, the latter being crucial for binding to the anionic sites of acetylcholinesterase. Six peptide sequences were synthesized based on the metal-binding sites, molecular mass, hydrophobicity, and bioactivity probability. Among the synthetic peptides, the VF dipeptide stood out both for its in vitro antioxidant and anticholinesterase activities. This peptide, as well as the fraction of Cu-binding peptides, should be further studied because it may act through different mechanisms related to neurodegenerative diseases, in addition to the chelation of the excess of metals in the central nervous system. (AU)

Processo FAPESP: 18/14818-9 - Estudo de alvos moleculares importantes para o controle do metabolismo em câncer: a via da mTOR/S6K com papel central
Beneficiário:Fernando Moreira Simabuco
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores - Fase 2
Processo FAPESP: 17/16977-4 - Obtenção e caracterização de peptídeos com atividade de neuroproteção
Beneficiário:Maria Elisa Caetano Silva Abreu
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/06457-0 - Folhas e talos de beterraba (Beta vulgaris L.): caracterização e efeitos da capacidade antioxidante em indivíduos obesos e dislipidêmicos
Beneficiário:Rosângela Maria Neves Bezerra
Linha de fomento: Auxílio à Pesquisa - Regular