| Texto completo | |
| Autor(es): |
Caetano-Silva, Maria Elisa
[1]
;
Simabuco, Fernando Moreira
[2]
;
Neves Bezerra, Rosangela Maria
[2]
;
da Silva, Daniele Cristina
[1]
;
Barbosa, Eder Alves
[3, 4]
;
Moreira, Daniel Carneiro
[4]
;
Brand, Guilherme Dotto
[3]
;
de Souza de Almeida Leite, Jose Roberto
[4]
;
Bertoldo Pacheco, Maria Teresa
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] ITAL, Inst Food Technol, Ctr Food Sci & Qual, CCQA, BR-13070178 Campinas - Brazil
[2] Univ Estadual Campinas, UNICAMP, Multidisciplinary Lab Food & Hlth, LABMAS, Sch Appl Sci, FCA, BR-13484350 Limeira - Brazil
[3] Univ Brasilia, Lab Synth & Anal Biomol, Inst Chem, IQ, BR-70910900 Brasilia, DF - Brazil
[4] Univ Brasilia, Fac Med, Morphol Area, Res Ctr Morphol & Appl Immunol, NuPMIA, UnB, BR-70910900 Brasilia, DF - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Agricultural and Food Chemistry; v. 68, n. 44, p. 12433-12443, NOV 4 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
This study aims to isolate metal-binding peptides and synthesize promising amino acid sequences to potentially act as neuroprotective compounds in the future, targeting different mechanisms. Fractions of whey metal-binding peptides (Cu, Fe, and Zn) isolated by immobilized metal affinity chromatography showed different amino acid profiles according to the metal. The Cu-binding peptides presented roughly twofold increase in the in vitro antioxidant, as assessed by oxygen radical absorbance capacity and anticholinesterase activities over the hydrolysate. This is probably because of the higher concentration of aromatic and basic residues, the latter being crucial for binding to the anionic sites of acetylcholinesterase. Six peptide sequences were synthesized based on the metal-binding sites, molecular mass, hydrophobicity, and bioactivity probability. Among the synthetic peptides, the VF dipeptide stood out both for its in vitro antioxidant and anticholinesterase activities. This peptide, as well as the fraction of Cu-binding peptides, should be further studied because it may act through different mechanisms related to neurodegenerative diseases, in addition to the chelation of the excess of metals in the central nervous system. (AU) | |
| Processo FAPESP: | 18/14818-9 - Estudo de alvos moleculares importantes para o controle do metabolismo em câncer: a via da mTOR/S6K com papel central |
| Beneficiário: | Fernando Moreira Simabuco |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2 |
| Processo FAPESP: | 17/16977-4 - Obtenção e caracterização de peptídeos com atividade de neuroproteção |
| Beneficiário: | Maria Elisa Caetano Silva Abreu |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/06457-0 - Folhas e talos de beterraba (Beta vulgaris L.): caracterização e efeitos da capacidade antioxidante em indivíduos obesos e dislipidêmicos |
| Beneficiário: | Rosângela Maria Neves Bezerra |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |